Network Method to Build Sample-Specific Glycan Database
A network method builds a sample-specific glycan database for N-linked glycosylation from MS/MS data.
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Glycans are critical for many biological processes, such as protein folding and intercellular communication
- About half of all mammalian proteins are glycosylated
- Mass spectrometry is widely used for studying proteins and glycoproteins
- Database search software is the predominant method for identifying proteins from tandem mass spectrometry data. Requirements are:
- Complete protein database — often satisfied due to the ease of genome sequencing
- For glycoproteins, additionally need complete glycan database — often not satisfied; usually only have imperfect knowledge
- Goal is to develop software that builds improved N-glycan databases by building a sample-specific glycan database based on the mass spectrometry data itself rather than relying solely on preexisting glycan databases.
