Comparative Analysis of Herceptin N-Linked Glycosylation by HILIC-FLD and LC- MS/MS Methods

Naveen Rauniyar from the Analytical Development Group at Tanvex BioPharma presents a study published in the Journal of Pharmaceutical and Biomedical Analysis, detailing a comparative glycan analysis of Herceptin using two methods: release glycan analysis with PLD and glycopeptide analysis with LC-MS/MS. The study is significant as it provides the first side-by-side comparison of these methods, emphasizing their complementarity in analyzing the glycosylation of monoclonal antibodies—a critical quality attribute in biopharmaceuticals. The research demonstrated strong correlation between the two techniques and highlighted their distinct advantages in detecting and quantifying glycan forms, including high-mannose, hybrid, and complex types, which play a crucial role in the biological efficacy of drugs.

This session was recorded in April 2024.

  • Understand the differences and synergies between PLD and LC-MS/MS in glycan analysis.
  • Assess the sensitivity and precision of each method in localizing and quantifying glycosylation.