Using the right practical tools, de novo sequencing now can be easily turned into an in-house, higher throughput procedure with extremely high confidence.
John Reed covers the ins and outs of PTM analysis. He touches on peptide identification and the use of standard curves and weighted averaging of XICs - and a little bit of ice cream.
With increasing complexity and volume of biotherapeutics, Janssen describe how they are implementing Byosphere to bring clarity to manage their workflows and deliver concise information when and where its needed.
The method utilizes ZipChip, a microfluidic capillary zone electrophoresis-based separation coupled with HRAM mass-spectrometry (CE-MS) for rapid characterization of oligos in positive ionization mode.
Bacterial microcompartments (BMCs) are widespread in bacteria and are used for a variety of metabolic purposes -authors used cross-linking probes to unravel the native assembly states of these large complexes.
For fast ranking during expression vector design and clone selection, Lonza's setup for high throughput intact MS uses Protein Metric’s parsimonious charge deconvolution algorithm.
The novel workflow for AdV VP analysis affords substantially lower analysis times than traditional peptide mapping methods, which require the use of several proteases and long sample processing for sufficient in-depth characterization.
A glycoproteomics platform comprising custom chemical probes and time resolved proteolysis of biogicals.
Microflow Liquid Chromatography Coupled to Mass Spectrometry (μLC–MS) Workflow for O-Glycopeptides Isomers Analysis Combining Differential Mobility Spectrometry and Collision Induced and Electron Capture Dissociation
Extending Proteome Coverage by Combining MS/MS Methods and a Modified Bioinformatics Platform Adapted for Database Searching of Positive and Negative Polarity 193 nm Ultraviolet Photodissociation Mass Spectra