Quick Guide to Peptide Analysis

HCP, HotSpot, PTM, SVA (C57) – Specific, SVA (C58) – Specific, S-S, MAM New Peak Detection, System Suitability, Oxidative Footprinting, and PTM (in-silico)

Peptide Analysis workflows have three separate tabs: Samples, Sequences, and Processing nodes.

NOTE: Considerations specific to the HCP and System Suitability workflows in the Sequences tab are called out.

Create a Workflow

Click to launch any of the mentioned workflows. In the figure below, the Post-Translational Modifications (PTM) workflow is highlighted.

Figure 1: Byos System Workflows with PTM highlighted

Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.

Figure 2: Drag and drop sample data file(s) to be processed into the Samples tab

Drag and drop the FASTA file to be processed into the Sequences tab.

Figure 3: Drag and drop FASTA file to be processed into the Sequences tab

NOTE: In the HCP workflow, the user must instead point to a protein database. The user can either drag and drop as shown in the figure below or direct Byos to a database file. This is done by clicking in the space to activate the light blue “…” box. The user is then prompted to select a file as shown in the figure below.

Figure 4: Click to activate the file path prompt

NOTE: In the System Suitability workflow, the Sequences tab is populated by default to include the Pierce RT standard. The user can replace this with another standard if desired. The user can change this by clicking to highlight the line and clicking Remove selection (shown in the figure below). Another protein can be brought in using drag and drop (shown in Figure 3).

Figure 5: The Pierce RT standard is included by default in the System Suitability workflow

Click Create Project to start processing and to generate the report.

Figure 6: Click Create Project.

The user will be prompted to name and save the project file. Please note Byos will save this type of project as a *.blgc file.

Figure 7: Name the project and save in a convenient location

Processing will begin. Once complete, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.

Figure 8: Workflows tab, completed project, and associated report are available as separate tabs