De Novo Sequencing of Monoclonal Antibodies

Quickly sequence hundreds or thousands of monoclonal antibodies (mAbs) by mass spectrometry with automatic assembly of individual peptide sequences into the full protein sequence

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De Novo Antibody Sequencing by Mass Spectrometry

The de novo sequencing workflow allows you to determine the sequence of unknown antibodies using only LC-MS/MS data. Antibodies are digested with multiple enzymes and the complex, intricate jigsaw is assembled in the correct order to generate a full antibody sequence.

Unique Algorithm

The workflow is unique in how it determines antibody sequences, mimicking the actual biological process of producing antibodies. The algorithm, in silico, converges on a final sequence in an iterative and completely automated process. The input is a raw MS file, and the output is the determined sequence with confidence metrics.

Unmatched Accuracy and Confidence

Provide high-confidence amino acid assignments, even for challenging residues like leucine and isoleucine. Visual inspection tools and intact mass analysis provide additional layers of confidence to your de novo sequencing results.

High Throughput, Automated Analysis

The de novo sequencing workflow runs 'hands-free,' automatically assembling individual peptide sequences into the full protein sequence. Results are displayed in a unique inspection framework so that scientists can review them efficiently prior to reporting out a validated sequence. This automated workflow allows for high-throughput data treatment and efficient analysis of large datasets.