SARS-CoV-2 Spike Ectodomain
This webinar covers HDX MS analysis of SARS-CoV-2 spike protein, focusing on glycopeptide inclusion to improve sequence coverage. It discusses techniques, results, and challenges, exploring potential for enhancing vaccines and treatments.
About This Webinar
Inclusion of Glycopeptides provides increased sequence coverage
Presented in October, 2023
Hydrogen/deuterium exchange mass spectrometry (HDX-MS) can provide precise analysis of a protein’s conformational dynamics across varied states, such as heat-denatured vs. native protein structures, localizing regions that are specifically affected by such conditional changes. Maximizing protein sequence coverage provides high confidence that regions of interest are located by HDX-MS, but one challenge for complete sequence coverage is N-glycosylation sites. The deuteration of peptides post-translationally modified by asparagine-bound glycans (glycopeptides) has not always been identified in previous reports of HDX-MS analyses, causing significant sequence coverage gaps in heavily glycosylated proteins and uncertainty in structural dynamics in many regions throughout a glycoprotein.
Key Learning Objectives
- Conformational dynamics of the spike glycoprotein from SARS-CoV-2 virus are analyzed and visualized
- The experimental workflow of hydrogen/deuterium exchange mass spectrometry is explained
- Adding glycopeptides from spike to analysis of its conformational dynamics improves detail in visualizations, and fills in coverage gaps reported by previous publications
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