Intact and Charge Variant Reconstruction

Combine the insights of intact mass and peptide mapping data for a comprehensive view of protein modifications. Obtain a more accurate and detailed understanding of the modifications present in your samples.

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Visualizing Modifications

The Intact Reconstruction workflow reconstructs a theoretical intact mass spectrum based on the quantified modifications from peptide mapping data and overlays it onto the experimental intact mass spectrum. This visualization enables direct comparison and confirmation of the identified modifications.

Enhanced Accuracy and Confidence

Reconstructing the intact mass spectrum using peptide-level data provides higher confidence in modification assignments and more accurate quantification of different protein forms. This level of detail is particularly useful in characterizing complex biotherapeutics.

Streamlined Workflow

Protein Metrics software automates the Intact Reconstruction process, minimizing manual intervention and simplifying the analysis. Researchers can easily export the necessary data from the peptide mapping project and import it into the intact project for reconstruction, making the workflow efficient and user-friendly.

Applications in Biotherapeutic Characterization

The Intact Reconstruction workflow finds wide applications in biotherapeutic characterization, particularly in analyzing antibody-drug conjugates (ADCs). The ability to accurately quantify modifications, like drug-to-antibody ratio (DAR), is crucial in assessing the quality and efficacy of ADCs.