HDX Mass Spectrometry Analysis

A vendor neutral solution for processing liquid chromatography–mass spectrometry (LC-MS) data acquired from hydrogen-deuterium exchange experiments

Effortless HDX Analysis

Characterize protein dynamics and interactions quickly and easily with Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS). Ready-made HDX workflows simplify the complex study of protein dynamics and interactions and offer automated solutions for oxidative labeling.

Deuterium Uptake Calculations Simplified

A single, streamlined approach enables identification and quantification of deuterium incorporation. Gain a complete picture of protein dynamics with an intuitive and easy-to-use visual interface that simplifies deuterium uptake calculations.

Streamlined Workflows and Enhanced Visualization

Scripts automate data processing, reducing HDX mass spec analysis time from days to hours. Minimize manual intervention and maximize efficiency.

A pre-loaded customizable report template contains a wide selection of calculations, plots and visuals (including volcano plots). View data concurrently in tabular form and on the 3D protein structure.

Explore Workflows

Peptide-Level Analysis

Identification of proteins and modified peptides in complex samples

Intact Analysis

Automatic MS/MS spectral annotation, MS level deconvolution, and identification

Oligonucleotides

Characterization and quantitation of oligos

Multi-Protein Quantitaion

Faster cell line selection, curated HCP quantitation, and instant protein mixture results

HDX Mass Spectrometry Analysis Resources

Dive into HDX MS analysis of SARS-CoV-2 spike protein, focusing on glycopeptide inclusion and its challenges.
Discover how automation enhances protein characterization, high-throughput MS, and data management with Byosphere.
The HDX tutorial series explains how to set up HDX workflows, analyze deuterium uptake, and generate detailed protein reports.
Explore higher-order structure in these videos on plasma-induced modification and bacterial microcompartment assembly insights.

Get started with Protein Metrics