Working with Peptide Workflows
Peptide workflows, from PTM and cross-link analysis to HCP and sequence variants. Learn how to create peptide projects, set up Byonic searches, interpret scores, validate peptides, manage disulfide mapping, and generate customized reports efficiently.
Series Content
Introduction to Peptide Workflows
INSIDE THE VIDEO
Get an overview of nine default peptide workflows in Byos and Byosphere, including PTM, HCP, cross-link, sequence variants, hotspots, and more.
LENGTH
2 mins
Creating Peptide Projects
INSIDE THE VIDEO
Learn how to create peptide projects in Byos by adding samples, editing sample types, and importing sequences. Simplify protease selection and alias naming.
LENGTH
2 mins
Introduction to Byonic Processing Nodes
INSIDE THE VIDEO
Learn how Byonic integrates into peptide and chromatography workflows in Byos and Byosphere, covering MS/MS identification, quantification, and node setup.
LENGTH
2 mins
Using the Byonic Processing Node
INSIDE THE VIDEO
Learn how to set up a Byonic search in Byos and Byosphere, including adding contaminants, setting digestion parameters, and using wildcard searches effectively.
LENGTH
8 mins
Overview to Byonic Results
INSIDE THE VIDEO
Learn how to interpret Byonic scores, Delta Mod scores, and validate peptide assignments using MS/MS fragmentation data.
LENGTH
3 mins
Using the Byologic Processing Node
INSIDE THE VIDEO
Learn to use the Byologic processing node: add peptides via theoretical digest or CSV import, adjust MS extraction settings, and enable advanced feature finder.
LENGTH
9 mins
Validation Comments and Label Overview
INSIDE THE VIDEO
Learn how to validate peptides and add comments and labels in project results. Customize views, filter data, and efficiently manage peptide annotations.
LENGTH
8 mins
Deamidation in the Peptide Workflow
INSIDE THE VIDEO
Learn how to inspect, adjust, and integrate deamidated peptides in PTM workflows. Split peaks, manage charge states, and exclude noise with ease.
LENGTH
4 mins
Overview of the Default PTM Report
INSIDE THE VIDEO
Learn how to generate and customize default PTM reports: modify tabs, apply filters, create templates, and export in various formats for streamlined analysis.
LENGTH
7 mins
Peptide Manager Explained
INSIDE THE VIDEO
Learn how to manage in silico peptides with Peptide Manager: import, merge, annotate, and track features in peptide workflows for streamlined analysis.
LENGTH
10 mins
IsoAsp in PTM Workflows
INSIDE THE VIDEO
Learn how to quantify aspartic acid isomerization with automated or manual methods, separate peaks, and analyze variants for accurate results.
LENGTH
4 mins
Learn More
Peptide How-Tos
Knowledge Base commonly asked questions about peptide workflows
Byonic: Focused Protein Databases
Application note focused on protein databases
Byonic: N-Linked Glycopeptide Analysis
Application note on N-linked glycopeptide analysis
Peptide Insights
A selection of Insight articles that cover topics on peptides
More Resources
GUIDEQuick Guide to Peptide Analysis
Getting started with HCP, HotSpot, PTM, SVA, S-S, MAM new peak detection, system suitability, and oxidative footprinting workflows.
VIDEO SERIESResearch Spotlight on Peptides
Watch these spotlight videos on peptides covering methods for mapping, quantitation, glycosylation, and antibody stability.
VIDEO SERIESWorking with Chromatogram Workflows
Learn to use chromatogram workflows for peak analysis, adjust baselines, integrate peaks, align traces, and explore glycan analysis.
VIDEO SERIESUsing Byonic
The Byonic tutorials explain MS/MS integration, peptide searches, charge adjustments, filtering PSMs, and report generation.