Working with Chromatogram Workflows
Learn to use chromatogram workflows for peak annotation, quantitation, PTM integration, and glycan analysis. Adjust baselines, integrate peaks, align traces, customize reports, explore glycan cartoons, and manage projects effectively.
Series Content
Intro to Chromatogram Workflows
INSIDE THE VIDEO
Learn how to use Byosphere's chromatogram-based workflows for peak annotation, relative quantitation, and trace comparisons across samples using dynamic time alignment.
LENGTH
2 mins
Adjust Peak Integration
INSIDE THE VIDEO
Learn how to adjust peak integration in Byosphere after project creation, including splitting, deleting, and adding peaks, to ensure accurate chromatogram analysis.
LENGTH
6 mins
Creating a MS2 Reference Project
INSIDE THE VIDEO
Create a reference chromatography project using MS/MS Byonic assignments, integrate PTM search results, and configure chromatogram settings.
LENGTH
3 mins
Creating In-Silico Reference
INSIDE THE VIDEO
Create an in silico reference project in Byosphere for peptide identification, peak annotation, and comparison using MS1 data and chromatogram traces.
LENGTH
5 mins
Creating a Comparison Project
INSIDE THE VIDEO
Create a comparison chromatography project in Byosphere to align and match chromatograms or electropherograms against a reference project for quantitative analysis.
LENGTH
3 mins
How to Manually Adjust Your Baselines
INSIDE THE VIDEO
Learn how to manually adjust the chromatograph baseline in Byosphere, add and move baseline anchors, and ensure accurate peak integration for quantitation.
LENGTH
3 mins
Using the Chromatogram Interface
INSIDE THE VIDEO
Navigate and label chromatogram projects with ease. Learn to manage reference and comparison workflows, zoom and filter traces, and flag peak area ratios efficiently.
LENGTH
6 mins
Processing Biophysical Non-MS Data
INSIDE THE VIDEO
Create reference and comparison chromatography projects for biophysical non-MS data. Learn how to add trace files, adjust peaks, and analyze peak ratios.
LENGTH
5 mins
Learn More
Chromatogram & Released Glycans
Commonly asked questions about chromatogram and released glycan workflows
Dynamic Columns
Insights article on dynamic columns, XIC area summed isoX normalized in Byos
Multi-Attribute Method (MAM)
Knowledge Base article on how to use the Multi-Attribute Method (MAM)
Validating Glycopeptide Results
Validating glycopeptide results, MS/MS filtering in Byonic
More Resources
GUIDEQuick Guide to Chromatogram Analysis
An easy guide to getting started with chromatography workflows, including reference, characterization and in-silico chromatography.
VIDEO SERIESWorking with Peptide Workflows
Explore peptide workflows, set up searches, interpret scores, validate peptides, manage disulfide mapping, and create reports.
VIDEO SERIESUsing Byonic
The Byonic tutorials explain MS/MS integration, peptide searches, charge adjustments, filtering PSMs, and report generation.
VIDEO SERIESUsing Byosphere
Byosphere enhances LC-MS analysis with custom searches, dashboards, automated workflows, audit trails, and project management.