Quick Guide to Chromatogram Analysis

Chromatogram Workflows

Chromatogram Analysis workflows have three separate tabs: Samples, Sequences, and Processing nodes.

Create a Workflow

Click to launch any of the above mentioned workflows. In the figure below, the Reference Chromatography workflow is highlighted.

Figure 1: Byos System Workflows with Reference Chromatography highlighted

Drag and drop the sample file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.

Figure 2: Drag and drop sample data files of interest into the Samples tab

The user can load a Traces file, an MS/MS search result *.byrslt file, and has an option to set the digestion parameters. To load the trace file and MS/MS search result file, click within the box to activate the light blue “…” box, and upload the respective files. For setting the digestion parameter, use the dropdown menu to select the enzyme.

Figure 3: Click to open file editors to load a trace file or an MS/MS search file ( *.byrslt)

The user can associate traces with samples. To associate traces, click within the box to activate the light blue “…” box, as shown in the figure below.

Figure 4: Associate traces with samples. Traces are assigned to all samples by default

Drag and drop the FASTA file to be processed into the Sequences tab.

Figure 5: Drag and drop FASTA file to be processed into the Sequences tab

Click Create Project to start analysis and report generation. Please note Byos will save this type of project as a *.bmap file.

Figure 6: Click Create Project.

The user will be prompted to name and save the analysis file.

Figure 7: Name the project and save in a convenient location

Processing will begin. Once complete, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.

Figure 8: Workflows tab, completed project, and associated report are available as separate tabs