Quick Start Guide to Intact Analysis
An easy guide to getting started with intact, reduced, ADC, intact reconstruction, and IntaBio icIEF-MS workflows.
Intact, Reduced, ADC, Intact Reconstruction and IntaBio icIEF-MS Workflows
The Intact Analysis workflows have four separate tabs: Samples, Sequences and masses, Sample-protein input, and Processing nodes.
Create a Workflow
Click to launch any of the mentioned workflows. In the figure below, the Intact workflow is highlighted.
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Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.
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Drag and drop the FASTA file to be processed into the Sequences and masses tab.
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Click Create Project to start processing and to generate the report.
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The user will be prompted to name and save the project file.
Please note Byos will save this type of project as a *.ntms file.
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Processing will begin. Once completed, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.
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Learn More
Intact How-Tos
Knowledge Base commonly asked questions about intact workflows
Workflow Setup for Low MW (<10 kDa) Isotopically Resolved Peptides
Knowledge Base article on setting up a workflow for low MW peptides
Intact Analysis of a Purified Therapeutic Protein Incorporating Top-Down Fragmentation
Application note for Intact analysis
Add an optical trace (UV, FL, etc.) to a project in Intact and Chromatogram workflows
Knowledge Base Article for Intact and Chromatogram workflows
More Resources
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VIDEO SERIESWorking with Intact Mass Workflows
Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
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VIDEO SERIESWorking with Reconstruction Workflows
Perform intact reconstruction and charge variant analysis. Set up projects, configure processing nodes, and align CIEF traces effectively.
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VIDEO SERIESResearch Spotlight on Intact Mass
Watch this video on a novel serum stability assay, enhancing accuracy in biologics screening with in vitro methods.
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PUBLICATIONLab of the Future: Fully Automated System for High-Throughput Mass Spec Analysis
A state-of-the-art, integrated, multi-instrument automated system for MS characterization of biotherapeutics.