Quick Start Guide to Intact Analysis

An easy guide to getting started with intact, reduced, ADC, intact reconstruction, and IntaBio icIEF-MS workflows.

Intact, Reduced, ADC, Intact Reconstruction and IntaBio icIEF-MS Workflows

The Intact Analysis workflows have four separate tabs: Samples, Sequences and masses, Sample-protein input, and Processing nodes.

Create a Workflow

Click to launch any of the mentioned workflows. In the figure below, the Intact workflow is highlighted.

Figure 1: Byos System Workflows. These are the default workflows included with installation

Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.

Figure 2: Drag and drop sample data file(s) to be processed into the Samples tab

Drag and drop the FASTA file to be processed into the Sequences and masses tab.

Figure 3: Drag and drop FASTA file to be processed into the Sequences and masses tab

Click Create Project to start processing and to generate the report.

Figure 4: Click Create Project.

The user will be prompted to name and save the project file.

Please note Byos will save this type of project as a *.ntms file.

Figure 5: Name the project and save in a convenient location

Processing will begin. Once completed, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.

Figure 6: Workflows tab, completed project, and associated report are available as separate tabs

Learn More

Intact How-Tos

Knowledge Base commonly asked questions about intact workflows

Workflow Setup for Low MW (<10 kDa) Isotopically Resolved Peptides

Knowledge Base article on setting up a workflow for low MW peptides

Add an optical trace (UV, FL, etc.) to a project in Intact and Chromatogram workflows

Knowledge Base Article for Intact and Chromatogram workflows

More Resources

Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
Perform intact reconstruction and charge variant analysis. Set up projects, configure processing nodes, and align CIEF traces effectively.
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