Quick Start Guide to Intact Analysis
An easy guide to getting started with intact, reduced, ADC, intact reconstruction, and IntaBio icIEF-MS workflows.
Intact, Reduced, ADC, Intact Reconstruction and IntaBio icIEF-MS Workflows
The Intact Analysis workflows have four separate tabs: Samples, Sequences and masses, Sample-protein input, and Processing nodes.
Create a Workflow
Click to launch any of the mentioned workflows. In the figure below, the Intact workflow is highlighted.
Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.
Drag and drop the FASTA file to be processed into the Sequences and masses tab.
Click Create Project to start processing and to generate the report.
The user will be prompted to name and save the project file.
Please note Byos will save this type of project as a *.ntms file.
Processing will begin. Once completed, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.
Learn More
Intact How-Tos
Knowledge Base commonly asked questions about intact workflows
Workflow Setup for Low MW (<10 kDa) Isotopically Resolved Peptides
Knowledge Base article on setting up a workflow for low MW peptides
Intact Analysis of a Purified Therapeutic Protein Incorporating Top-Down Fragmentation
Application note for Intact analysis
Add an optical trace (UV, FL, etc.) to a project in Intact and Chromatogram workflows
Knowledge Base Article for Intact and Chromatogram workflows
More Resources
VIDEO SERIESWorking with Intact Mass Workflows
Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
VIDEO SERIESWorking with Reconstruction Workflows
Perform intact reconstruction and charge variant analysis. Set up projects, configure processing nodes, and align CIEF traces effectively.
VIDEO SERIESResearch Spotlight on Intact Mass
Watch this video on a novel serum stability assay, enhancing accuracy in biologics screening with in vitro methods.
PUBLICATIONLab of the Future: Fully Automated System for High-Throughput Mass Spec Analysis
A state-of-the-art, integrated, multi-instrument automated system for MS characterization of biotherapeutics.