Working with Intact Mass Workflows
Learn to run intact mass workflows in Byos, manage raw data, protein sequences, delta masses, and deconvolution settings. Generate reports for ADCs, glycosylation, and oligonucleotide analysis with custom workflows and auto-assigned protein masses.
Series Content
Introduction to Intact Mass Workflows
INSIDE THE VIDEO
Learn to run intact mass workflows with Byos One Touch. Add raw data, adjust settings, and generate reports. Supports ADC, native MS, and glycosylation analysis.
LENGTH
4 mins
Adding Data and Peaks Integration
INSIDE THE VIDEO
Learn to add MS raw data and set integration settings in Byos Intact Mass workflow. Drag/drop files, adjust integration types, and define time windows.
LENGTH
3 mins
Adding Protein Sequences
INSIDE THE VIDEO
Learn to add protein sequences in Byos' intact mass workflow. Drag/drop FASTA files, create antibodies, and manage impurities or protein complexes efficiently.
LENGTH
5 mins
Managing Delta Mass
INSIDE THE VIDEO
Manage delta masses in Byos' intact mass workflow. Add manually, import via CSV, or create combinations for multiple PTMs or modifications using the combination tool.
LENGTH
4 mins
Managing Deconvolution Settings
INSIDE THE VIDEO
Learn how to manage deconvolution settings in Byos' intact mass workflow. Adjust mass ranges, peak detection, and handle isotopic data for comprehensive analysis.
LENGTH
3 mins
Using the GUI for Intact Mass
INSIDE THE VIDEO
Overview of Byos' intact mass workflow interface. Navigate sample files, visualize peaks, adjust chromatograms, and link deconvoluted spectra to TIC/MS1.
LENGTH
4 mins
Using CSV Workflows
INSIDE THE VIDEO
Learn how to perform batch analysis using an intact mass flow. Import a CSV file through the Sample Protein Input tab, essential for populating the sample MS raw data file.
LENGTH
3 mins
Overview to Intact Mass Reports
INSIDE THE VIDEO
Watch the video to learn how to generate reports using various templates and customize them with charts, normalization calculations, and source summaries in Byos.
LENGTH
6 Minutes
Using the ADC Workflow
INSIDE THE VIDEO
Learn how to use the ADC workflow for drug-to-antibody ratio (DAR) analysis. Set sequences, modify Delta Masses, and generate normalized intensity reports.
LENGTH
3 mins
Learn More
Intact How-Tos
Knowledge Base commonly asked questions about intact workflows
Workflow Setup for Low MW (<10 kDa) Isotopically Resolved Peptides
Knowledge Base article on setting up a workflow for low MW peptides
Intact Analysis of a Purified Therapeutic Protein Incorporating Top-Down Fragmentation
Application note for Intact analysis
Add an optical trace (UV, FL, etc.) to a project in Intact and Chromatogram workflows
Knowledge Base Article for Intact and Chromatogram workflows
More Resources
GUIDEQuick Start Guide to Intact Analysis
An easy guide to getting started with intact, reduced, ADC, intact reconstruction, and IntaBio icIEF-MS workflows.
VIDEO SERIESResearch Spotlight on Intact Mass
Watch this video on a novel serum stability assay, enhancing accuracy in biologics screening with in vitro methods.
VIDEO SERIESUsing Byonic
The Byonic tutorials explain MS/MS integration, peptide searches, charge adjustments, filtering PSMs, and report generation.
WEBINARIntact Characterization of Multispecifics
Learn about multispecific antibodies and the use of 2D LC-MS for improved production and analysis.