Working with Digested Oligo Workflows
The Byos Digested Oligonucleotide tutorial series covers workflow setup, modification configuration, investigation view navigation, layout customization, oligonucleotide tables, and creating report templates for sequence coverage and abundance analysis.
Series Content
Introduction to Digested Oligo Workflow
INSIDE THE VIDEO
Review the Byos Digested Oligonucleotide workflow for RNase digests. Learn sequence database searching, quantitation, and custom reporting.
LENGTH
2 mins
Setting up Digested Oligos Workflow
INSIDE THE VIDEO
Set up workflows for Byos Digested Oligonucleotide analysis. Add files, define sequences, set digestion and modification parameters.
LENGTH
9 mins
Modifications for Digested Oligos
INSIDE THE VIDEO
Learn how to configure modification settings in the Byos Digested Oligonucleotide workflow. Adjust 5' and 3' ends, and search for specific oligo modifications.
LENGTH
7 mins
Investigation View for Digested Oligos
INSIDE THE VIDEO
Learn how to use the Byos Digested Oligonucleotide Project Investigation View. Review tools, tables, and plots to explore and validate your project data.
LENGTH
6 mins
Table View for Digested Oligos
INSIDE THE VIDEO
Learn how to navigate the oligonucleotide table in Byos, from sample and charge state organization to using the validate, score, and delta score columns effectively.
LENGTH
14 mins
Reporting for Digested Oligos
INSIDE THE VIDEO
Learn how to create custom report templates for oligonucleotide analysis in Byos. View sequence coverage, abundance graphs, and export your reports efficiently.
LENGTH
3 mins
Customizing Layout for Digested Oligos
INSIDE THE VIDEO
Learn how to customize and save project layouts in Byos. Adjust pane sizes, columns, and decimal places, then save and load layouts for future use.
LENGTH
5 mins
Learn More
Workflow How-Tos
Knowledge Base how to guides for using a variety of workflows
Digested Oligonucleotides Workflow
Knowledge Base article focussing on the digested oligonucleotides workflow
Oligonucleotide and Impurity UV-MS Quantitation
Insights article on oligonucleotide and impurity UV-MS quantitation
Oligonucleotide Building Blocks Basics
Insights article covering the basics of oligonucleotide building blocks
More Resources
GUIDEQuick Guide to Oligonucleotide Analysis
Learn how to get started with oligonucleotide analysis, including digested oligonucleotide analysis.
VIDEO SERIESWorking with Intact Mass Workflows
Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
VIDEO SERIESUsing Byonic
The Byonic tutorials explain MS/MS integration, peptide searches, charge adjustments, filtering PSMs, and report generation.
WEBINARMS Analysis of Intact Oligonucleotides
Explore advanced workflows for oligonucleotide analysis, focusing on fragmentation and MS2 annotation.