Quick Guide to Oligonucleotide Analysis

Learn how to get started with oligonucleotide analysis, including digested oligonucleotide analysis.

Oligonucleotide Workflow

Users can input MS1 data of Oligonucleotides to identify different molecules within the sample. Byos software has a novel algorithm that deconvolves the Oligonucleotide data to generate the mass spectrum.

The Oligonucleotide analysis project files are stored as *.olms files. An additional workflow has been added in Byos 5.4 that supports Digested Oligonucleotide analysis. Please see the Oligo User Guide for more information.

Figure 1: Oligonucleotide Deconvolved Mass data analysis

Learn More

Workflow How-Tos

Knowledge Base how to guides for using a variety of workflows

Digested Oligonucleotides Workflow

Knowledge Base article focussing on the digested oligonucleotides workflow

Oligonucleotide and Impurity UV-MS Quantitation

Insights article on oligonucleotide and impurity UV-MS quantitation

Oligonucleotide Building Blocks Basics

Insights article covering the basics of oligonucleotide building blocks

More Resources

Learn Byos Digested Oligonucleotide workflows, modification setup, investigation tools, and custom report creation.
Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
The Byonic tutorials explain MS/MS integration, peptide searches, charge adjustments, filtering PSMs, and report generation.
Explore advanced workflows for oligonucleotide analysis, focusing on fragmentation and MS2 annotation.

Get started with Protein Metrics