Quick Guide to De Novo Sequence Analysis

De novo Workflow

The de novo sequencing workflow has the Samples and Processing nodes tabs.

Create a Workflow

Click to launch the de novo sequencing workflow.

Figure 1: Byos System Workflows with de novo sequencing highlighted

Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.

Figure 2: Drag and drop sample data files of interest into the Samples tab

[OPTIONAL] Specify an alternative Byonic parameter file (*.byparms) by clicking within the box to activate the light blue “…” box, as detailed in the figure below.

Figure 3: Click to activate the Alternative Byonic Parameter File and select the *.byparms file to be applied during processing

[OPTIONAL] If a starting sequence template is to be used, paste the Heavy Chain and Light Chain sequences.

Figure 4: The sequences for the Heavy Chain and Light Chain can be pasted in as text.

Click Create Project to start analysis and report generation. Please note Byos will save this type of project as a *.blgc file.

Figure 5: Click Create Project.

Name the project and save in a convenient location.

Figure 6: Name the project and save in a convenient location

Processing will begin. Once complete, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.

Figure 7: Workflow tab, completed project, and associated report are available as separate tabs