Quick Guide to Charge Variant Analysis
Follow this short guide and learn how to get started with change variant analysis with reconstruction.
Charge Variant Analysis with Reconstruction Workflow
A new multi-node workflow using Byonic, Byologic, and Byomap has been added to Byos to support Charge Variant Analysis with Reconstruction.
Create a Workflow
Within Project Creation within the workflow, users should add an MS file and pI file in their own row entry, as well as the relevant FASTA file(s). Within the processing node, check/change the following settings: Make sure the Sample Names for the Byonic and Byologic nodes are reading the proper MS file.
Attach the report template Blgc_Reconstruction_export.rptc within the Byologic node and create project.
There should be two projects automatically created with the same filename, one .blgc and one .bmap. Both projects should automatically generate reports. The Report for the blgc project should be the one that was generated with the defined report template.
Learn More
Byos General
Knowledge Base general articles for using Byos
Intact Reconstruction Workflow
A Knowledge Base how to guide for using intact reconstruction workflows
Charge Variant Analysis with Reconstruction How-To
Knowledge Base article on how to do charge variant analysis with reconstruction
Intact Deconvolution With Isotopic Resolution
Intact deconvolution with isotopic resolution, intact reconstruction in Byos
More Resources
VIDEO SERIESWorking with Reconstruction Workflows
Perform intact reconstruction and charge variant analysis. Set up projects, configure processing nodes, and align CIEF traces effectively.
VIDEO SERIESUsing Byosphere
Byosphere enhances LC-MS analysis with custom searches, dashboards, automated workflows, audit trails, and project management.
WEBINARReconstructing cIEF Profiles Using Peptide Mapping Data
This webinar explores cutting-edge methods for reconstructing cIEF profiles using peptide mapping data with a focus on practical applications.
PUBLICATIONOrthogonal Comparison of Analytical Methods by Theoretical Reconstruction from Bottom-up Assay Data
Bottom-up methods like peptide mapping can be used to build reconstructions of both theoretical intact mass spectra and theoretical electropherograms.