Quick Guide to HDX Workflows
An easy guide to getting started with HDX analysis, including all our HDX workflows.
Hydrogen-Deuterium Exchange (HDX) Workflow
The HDX workflow has three separate tabs: Samples, Sequences, and Processing nodes.
Create a Workflow
Click to launch the workflow. In the figure below, the Hydrogen-Deuterium Exchange (HDX) workflow is highlighted.
Drag and drop the sample data file(s) to be processed into the Samples tab, as shown in the figure below. These can be from any vendor.
Drag and drop the FASTA file to be processed into the Sequences tab.
Click Create Project to start processing and to generate the report.
The user will be prompted to name and save the project file. Please note Byos will save this type of project as a *.blgc file.
Processing will begin. Once complete, the project and associated report will appear as additional tabs after the Workflows tab, as shown in the figure below.
Learn More
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Knowledge Base general articles for using Byos
Protein 3D File (pdb) Configuration
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How To Set MSMS Digestion Search Using Byonic
Knowledge Base article on how to set MSMS digestion search using Byonic
Integrating Byos with GraphPad Prism for Comprehensive Visual Analysis
Application note for Byos and GraphPad Prism integration
More Resources
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Explore higher-order structure in these videos on plasma-induced modification and bacterial microcompartment assembly insights.
VIDEO SERIESWorking with Hydrogen Deuterium Exchange (HDX)
The HDX tutorial series explains how to set up HDX workflows, analyze deuterium uptake, and generate detailed protein reports.
VIDEO SERIESUsing Byosphere
Byosphere enhances LC-MS analysis with custom searches, dashboards, automated workflows, audit trails, and project management.
WEBINARSARS-CoV-2 Spike Ectodomain
Dive into HDX MS analysis of SARS-CoV-2 spike protein, focusing on glycopeptide inclusion and its challenges.