This webinar covers the advanced analysis of erythropoietin (EPO) using Zip Chip capillary electrophoresis and Protein Metrics software, emphasizing the intact mass workflow. Marshall Bern explains how the data from 908 Devices is utilized to explore the intricacies of EPO analysis, highlighting the capabilities of the intact mass software. He shows the process of dividing mass spectrometry data into time slices, illustrating how different proteoforms are detected and analyzed over specific intervals, and introduces the latest features in the software, such as time traces for selected masses, enhancing the accuracy of data interpretation.

In the second part, Marshall provides a detailed explanation of methodologies for creating projects in the software, including automating data import and defining protein sequences and complexes. He discusses the challenges and solutions in setting up glycoform combinations for EPO, demonstrating the software’s ability to handle complex glycosylation patterns with minimal false annotations. The session also covers the algorithm for charge deconvolution, showcasing its robustness in differentiating between charge states and validating results using the new mass XIC feature. He compares the data with published results, reinforcing the reliability and in-depth of analysis provided by the software, making it an invaluable tool for researchers working with glycoproteins.