By using multiple proteases and dual fragmentation the authors are able to get the most accurate sequences and the best depth of coverage whilst optimizing protocols for de novo antibody sequencing
John Reed covers the ins and outs of PTM analysis. He touches on peptide identification and the use of standard curves and weighted averaging of XICs - and a little bit of ice cream.
The cell and developability sciences group is tasked with delivering lead manufacturing cell lines. Not only are the volume of request increasing, but so are the novelty and complexity of new molecules to characterize.
Unlock the complexities of Bispecific Analysis and immediately share simple to understand visualizations with others? That's exactly what Janssen's cell line development group have done. Come and find out how.
Marshall Bern details the usage of Intact Mass deconvolution in order to tease apart the complex glycosylation profiles of EPO
As minor changes to production processes can influence both glycan structure and composition, glycans must be monitored. However, characterization of N-linked glycosylation is analytically challenging due to innate glycan microheterogeneity.
The novel workflow for AdV VP analysis affords substantially lower analysis times than traditional peptide mapping methods, which require the use of several proteases and long sample processing for sufficient in-depth characterization.
A new experimental set up utilizing a multi enzyme approach and the best approaches for quantification
Microflow Liquid Chromatography Coupled to Mass Spectrometry (μLC–MS) Workflow for O-Glycopeptides Isomers Analysis Combining Differential Mobility Spectrometry and Collision Induced and Electron Capture Dissociation
Extending Proteome Coverage by Combining MS/MS Methods and a Modified Bioinformatics Platform Adapted for Database Searching of Positive and Negative Polarity 193 nm Ultraviolet Photodissociation Mass Spectra