Lysine-based conjugation is a widely used non-specific conjugation strategy for the development of Antibody Drug Conjugates (ADCs). Conjugation at lysine residues results in highly heterogeneous products that pose challenges to current characterization methods.

The Byos platform Intact workflow offers efficient identification, inspection, and relative quantification of charge variants, while reliably detecting even the low abundance species and highlighting even subtle differences between innovator and biosimilar products.

Here we describe a state-of-the-art, integrated, multi-instrument automated system designed to execute methods involved in mass spectrometry characterization of biotherapeutics, as implemented at Janssen.

The Byos platform Intact workflow offers efficient identification, inspection, and relative quantification of charge variants, while reliably detecting even the low abundance species and highlighting even subtle differences between innovator and biosimilar products.

The Byos HDX workflow makes it easy to analyze complex deuterium ratio calculations and visualize the results, helping to reduce analysis time

Authors report how PLIMB can be used to understand potential mechanism for bsAb aggregate formation using a tool molecule from clarified culture harvest

Raw MS data and associated meta data are swept to an automation module in Byosphere that resides in the J&J cloud server.
Advanced querying capabilities and quality control of data allows triaging voluminous data sets

Here we describe a state-of-the-art, integrated, multi-instrument automated system designed to execute methods involved in mass spectrometry characterization of biotherapeutics, as implemented at Janssen.

Combining OBE nMS with PMI software enables rapid analysis of masses, binding partners, and stoichiometries of designed protein assemblies

Study of anti-spike protein (SARS-CoV-2) blocking mAbs with a focus on measuring Fab glycosylation and its impact on binding to spike protein antigens

Monosaccharide composition is often discernible directly from accurate molecular mass (MS1); however, topology (monosaccharide connectivity) requires MS2, and glycosidic linkage information and anomericity requires, at least, MS3 if not combined with orthogonal insight.1 Here we report on disambiguation of topological isomers directly from tandem MS spectra