This talk covers the intricate analysis of glycoproteins, emphasizing the enhancement of glycoprotein dimensionality, and challenges in isomer quantification. Karli Reiding, an assistant professor from Utrecht University, provides insights into recent work on glycoprotein analytics. Discussed is the structural visualization of glycans on proteins, highlighting variations in glycosylation and its implications for protein stability and functionality. The presentation delves into different glycan species and their compositional analysis, elaborating on methodologies for measuring and enriching glycoprotein samples. This includes techniques like hydrophilic enrichment and LC-MS workflows, which are crucial for detailed glycopeptide analysis.

Furthermore, the Karli explores the use of advanced mass spectrometry and ion mobility spectrometry to analyze glycopeptides, emphasizing the importance of optimizing collision energies to balance the detection of glycan and peptide information. Findings of these distinct glycosylation patterns in neutrophils compared to plasma proteins, underscores the biological significance of these variations.