This webinar covers the topic of impurity characterization using modified siRNA, presented by Simon Gammel Gard from Novo Nordisk. Simon, a research scientist in the CMC Analytical Support Department, discusses the workflow for analyzing oligonucleotides and their impurities using LC-MS. The workflow, an adaptation of the intact module used for peptides and proteins, allows for the recognition of the primary structure of oligonucleotides, enabling automated mass calculation and sequencing. Simon also explains the therapeutic mechanism of siRNA in targeting and silencing specific proteins.

The presentation delves into the challenges of reversed-phase chromatography and the necessity of ion-pairing agents. Software is critical in speeding up data analysis and ensuring accurate impurity characterization.