Digested mRNA Analysis of Sequence, 5’ Cap, and PolyA Tail

Webinar Summary

Discover a unified approach to mRNA PQA analysis with LC-MS/MS and advanced data processing in Byos. This webinar will showcase how enzymatic digestion, combined with ion-pair reversed-phase separation, enables in-depth profiling of mRNA sequence, 5’ cap structures, and PolyA tails. Using real-world data from eGFP mRNA, we’ll demonstrate how to tackle isomer resolution, quantify capping efficiency, and deconvolute PolyA length variants—all within a streamlined, high-coverage workflow.

Key Learning Points

• Learn how digested oligonucleotide mapping provides >99% sequence coverage for mRNA, even in the presence of isomeric oligos.
• Understand how to quantify 5’ cap variants and intermediates using Byos software and robust MS/MS scoring techniques.
• See how PolyA tail deconvolution and mass matching reveal length distributions with LC-resolved detail for high-confidence PQA assessment.