Generating intact protein charge envelopes in mass spectrometry requires careful sample prep, often excluding reagents commonly used to prevent non-specific analyte interactions with vessels. Examples include salts (NaCl, KCl, MgCl2) and detergents (NP-40, SDS, Tween-20), which can suppress signals or complicate spectra.

MOBILion Systems developed a high-resolution ion-mobility system that separates compounds by collisional cross section (CCS) post-ionization, aiding intact protein analysis. This seminar will discuss how combining ion-mobility with Protein Metrics HRIM software enhances data processing and mass spectral accuracy.