Challenges and Strategies in Glycan Analysis by MAM

About This Poster

Multi-Attribute Method (MAM) presents unique advantages in glycan analysis for biotherapeutics over HILIC released glycan analysis. Some of the advantages include site-specific characterization and quantitation of glycans and non-glycosylation levels in a single assay and the relative ease of sample preparation without additional labeling steps. However, it is still challenging to match the relative quantities from both techniques often due to large differences in size and ionization efficiencies of glycan containing peptides. This poster aims to describe and demonstrate the effects of various parameters on the relative quantitation results.