Real-Time Informatics Pipeline for Biologics Screening During Expression
A real-time informatics pipeline enables the screening of biologics during high-throughput expression.
About This Poster
High Throughput (HT) Mass spectrometry (MS) has been indispensable to the discovery of small molecules, peptide/mini protein drug modalities, providing accurate molecular masses with very high accuracy and speed. HT ESI-MS of antibodies was recently demonstrated by Sawyer et al., PNAS, 2020, 117, 9851-9856. Motivated by this work, we have developed a complete end-to-end (E2E) pipeline coupling RapidFire-MS (RF-MS) data acquisition with real-time Informatics and data aggregation for biologics. We have successfully deployed this E2E workflow to identify hundreds of large molecules in a day with minimal human intervention enabling unprecedented insight into the quality of biologics in real-time at HT screening scale. As a result, we enable the integration of production, analytics, and informatics to screen large panels of biologics, e.g., >103-104 molecules, by streamlining hardware and data automation. We demonstrate the analytical figures of merit of how pipeline delivery of biologics compares to traditional analytical and off-the -bench informatics solutions.
