Reconstructing cIEF Profiles Using Peptide Mapping Data in Byos

with Bo Zhai, Sr Principal Scientist, Johnson and Johnson

Biopharmaceuticals often show variability in their biochemical properties due to posttranslational modifications and degradation events. Capillary Isoelectric Focusing (CIEF) is a critical technique used to assess charge heterogeneity, a key quality attribute if charge variants are known to affect the product’s safety, efficacy, or stability. While identifying the main species is straightforward, isolating and characterizing acidic and basic variants remains challenging. Through advancements in peptide mapping, we can now offer deeper insights into these variations and their impact on biopharmaceutical development.

In this session, we will showcase an enhanced Byos workflow that reconstructs CIEF profiles from peptide mapping data, offering a clearer understanding of charge heterogeneity. This updated workflow accounts for specific modifications like proline amidation and glutamate cyclization, enabling rapid and accurate profile comparisons.

Register now to explore how this innovative approach can streamline your process development and stability studies!