What's New in Byonic, Byos and Byosphere 4.2

New Features

• New Byos workflow: Oligonucleotide analysis. MS1-based analysis with MS2 Fragmentation viewer
• New Byos workflows: HRIM Intact, HRIM Peptide, and HRIM Glycan for MOBILion High-Resolution Ion Mobility (HRIM) analysis
  • HRIM Intact workflow to support intact analysis of MOBILion HRIM data
  • HRIM Peptide workflow to support peptide analysis of MOBILion HRIM data
  • HRIM Glycan workflow to characterize released glycan analysis of MOBILion HRIM data
• Support for simultaneous C-Term and N-Term specific enzymes (e.g., LysC and AspN)
  • Peptide and Chromatogram Analysis can now support a combination of C-Term and N-Term proteases
• Advanced feature for grouping in-silico peptides from CSV file with MS/MS peptides
  • In the previous releases, peptides from MS/MS identification and peptides imported from a CSV file (in-silico assignments) were grouped separately. These peptides are now grouped together if the retention times of the peptide from MS/MS and in-silico assignments are same
• HDX – New signal processing parameters to improve the sensitivity of HDX analysis
• MS/MS annotation
  • Improved oxonium ion annotation for ECD MS/MS spectra
  • Improved MS/MS annotation at lower m/z regions ( < 500 m/z )
• Processing – File Reading
  • Bruker – improved analysis with newer chromatogram acquisition of Total Ion Current (TIC) and Base Peak Intensity (BPI) traces
  • Thermo Scientific, Waters, and SCIEX instruments – metadata information such as Vendor, Model, Run date and other fields such as liquid chromatography (LC) setup used for the experiment can now be accessed in Byos
  • Shimadzu – Byos software can now read, parse and analyze the Shimadzu *.lcd SP2 data
• Reporting – Visualization
  • Add images to reports – drag and drop an image into the free text in a report or the template editor in the summary tab
  • Add a predefined pivot table within a new plots tab
  • Undetected peptides – now annotated in Peptide and Chromatogram Analysis reports through a new label “ND” in the Peak Comment column
  • Duplicate pivot table headers on every page
  • Export images in SVG format
  • Enhanced Excel exporting – merge pivoted rows and columns, display every row and column for each record, and show report details
• Reporting – Plots and Charts
  • Stacked plots – ability to show/hide stacked plots in the report
  • Show correlation percentage in PCA plot
  • Rotate labels in error bar and line charts
  • Add multiple pie charts in the same window/page
• Reporting – Disulfide linkages
  • Improved Xlink Class column ordering of disulfide linkages – the Xlink Class column “Expected” will now be ranked higher than other alternatives for the same peptide
  • Improved reporting for disulfide linkages within the same protein – the Xlink Partner Peptide Protein Name now reports the Protein name for disulfide linkages within the same protein