Intact Reconstruction for Bi-Specific Antibody Characterization
In this study, we utilize intact reconstruction to integrate tryptic digest peptide data with intact mass spectrometry (MS) data using a newly developed workflow to quickly characterize bsAbs.
About This Poster
Utilization of Intact Reconstruction for Bi-Specific Antibody Characterization
Bi-specific antibodies (bsAbs) represent an important expansion of biotherapeutics. With their ability to target multiple epitopes or antigens, bsAbs are an emerging biotherapeutic to treat rapidly changing and complex diseases such as cancer while maintaining their effectiveness compared to traditional treatments. With this ability comes unique challenges to characterize the final constructs from manufacturing. The propensity for mispairing chains, complex glycan profiles, and additional post translation modifications (PTMs) lead to a large number of similar masses that traditionally require several different approaches to properly characterize the bsAbs. In this study, we utilize intact reconstruction to integrate tryptic digest peptide data with intact mass spectrometry (MS) data using a newly developed workflow to quickly characterize bsAbs.
Our intact reconstruction workflow rapidly characterizes bispecifics, identifying chain mispairing, glycan profiles, and PTMs to streamline accurate bispecific analysis.
