Ion mobility has evolved and this poster shows how much higher resolution can help with deeper characterization of biotherpeauics. Usies the HRIM workflows specific to Mobilion systems.

6-Jun-22 Poster MP 317

Ion mobility is applied to characterization at the intact, subunit and peptide levels, enabling much faster LC runs and the ability to separate near-isobaric and isomeric species faster than befoe. Poster by Mobilion.

10-Jun-22 Poster ThP 409

For protein biotherapeutics, the generation of a manufacturing cell line to support product development is critical. The product quality of hundreds of cell-lines per program are screened by mass-spectrometry (MS). However, the nature of MS data impedes the implementation of end-to-end automation of analysis. Here, we describe the design and implementation of automated workflows for intact MS data processing and reporting.

7-Jun-22 Poster TP 211

Janssen has developed an end-to-end solution for the support of cell line development. Along with a Thermo mass spectrometry platform and robotics that feed Byos software, Hirsh Nanda's lab is able to truly implement the screening of hundreds of cell lines from bioreactors in less time than before. See the automation in action at Thermo's user meeting in the 'From Discovery to QC' section

Hyatt Regency Minneapolis - Nicollet D 10:25 - 10:45 a.m. [CDT]

Complex milk glycoproteins classified and analysed by LCMS to work out which ones survive infant digestion. Byos used to calculate relative abundances and types of glycosylations.

8-Jun-22 WP 225

MS^n capability on O-linked Glycans showing the capability of annotating fragments correctly, automatically in the spectrum. Use of glycan cartoons for reporting. Annotation of fragmentation automated in the software.

8-Jun-22 Poster WP 201

Complex glycoprotein analysis: Glycosylation, including glycan structures and occupancy, plays a vital role in the stability and efficacy of protein-based vaccines. Here, the ability of latest ECD technology to characterize a vaccine protein with multiple glycosylation sites was examined. Poster with Janssen and Sciex

9-Jun-22 Poster ThP 185

Deep search of the glycoproteome is an important element to understand disease states, and this is illustrated by the work on glioblastomas by this lab in Astrazeneca at the forefront of research.

9-Jun-22 ThOF pm 02:50

O-linked glycoslyations captured in a comprehensive analysis using alternative fragmentation techniques in combination with differential ion mobility. Protein Metrics software annotates the fragments and localises the O-glycosylation

9-Jun-22 ThP 267

Disulfide bond mapping is notoriousy challenging and in protein characterization has often required numerous experiments. Here, Agilent and e-Msion use ECD technology to use CID and ECD together, and annotate the spectra with Byos software.

7-Jun-22 Poster TP 426

Characterization of disulfides and trisulfide bonds is challenging and for bispecific antibodies the level of complexity is even higher. ECD fragmentation is supported in Byos and MS/MS spectra obtained by ECD provided a much higher level of sequence information compared to CID.

9-Jun-22 Poster ThP 408

Using proteomics analysis, UC Davis researchers looked at the anitcancer compounds associated with tropical fruits and looked at how they inhibit mitochondrial and energy production processes in human non-small cell lung carcinoma. Byos software helped analye the results

8-Jun-22 Poster WP 070

Using an emerging technique for epitope mapping, and intelligent use of radicals, Immuto have stdied the binding of the HER2 receptor to Trastuzumab right down to the residue level.

6-Jun-22 Poster MP 025

Accurately mapping deuterium locations in HDX relies partially on tracking deuterium during the chromatography separation which have had to be swift in the past. This study uses time-warping in long LC-MSMS gradients with Byos software to map back to HDX-MS experiments, and therefore allow the study of larger complexes.

7-Jun-22 Poster TP 162

For epitope mapping, reaching the residue level is challenging - and with Plasma Induced Modification of Biomolecules (PLIMB) new gains can be made. PLIMB irreversibly labels solvent accessible amino acid residues and this study demonstrates enhanced labeling coverage.

9-Jun-22 Auditorium -ThOE 2:50 pm - 3:10pm

How does the a triggering receptor bind to its ligands in the TREM2 Myeloid cells? The WashU lab looked at thjs with Fast oxidative footprinting of proteins

9-Jun-22 Poster ThP 420

Native MS and hydroxyl radical footprinting are used together by the Washington University lab to study the Ebola virus' structure and function.

9-Jun-2022 Poster ThP 452

This talk at the Agilent user meeting shows how new, innovative techniques can be used to much more efficiently characerize therapeutic proteins

5-Jun-22 Oral 12:30 PM - 3:00 PM

Deconvolution of Protein therapeutics acquired in native or denatured conditions from different high resolution mass spectrometers.

6-Jun-22 Oral Room L100 (8:50 am -9:10 am)

New algorithms for the deconvolution of new modalities demonstrate how important it is to leverage all possible pieces of inormation. In this poster, in collaboration with Janssen, Ignat Shilov from Protein Metrics illustrates that even low abundance signals can be effectively used.

7-Jun-22 Poster TP 024

Great innovative use of microdroplet technology, ion mobility and ECD to tackle subunit characterization in one go. Oral by Agilent, in collaboration with Janssen and eMSio, using Byos software for data analyis.

10-Jun-22 Oral Auditorium ThOE 10:10am - 10:30am (Central)

Adeno-associated viruses (AAVs) are a crucial tool for development and delivery of cutting-edge gene therapies. We developed liquid chromatography mass spectrometry (LC-MS) methods for AAV characterization for host cell protein analysis (HCP) and sequence variant analysis (SVA). In addition, orthogonal methods of subunit and peptide mapping were compared directly to capture discrepancies.

This vendor agnostic protocol requires no method development, can be easily adopted for Oligos characterization workflows such as intact mass analysis, analysis of impurities and degradation products.

Poster ThP 361

The use of a 908Devices ZipChip coupled to a high resolution mass spectrometer as an orthogonal method to chromatography for oligonucleotide analysis.

8-Jun-22 Poster WP 396