In this webinar, Wendy Hale from Agilent Technologies and Michelle English from Protein Metrics l delve into the analysis of Adeno-associated virus (AAV) using liquid chromatography-mass spectrometry (LC-MS), focusing on the critical quality attributes of viral capsid proteins. Participants will be introduced to the basics of AAVs and the LC-MS technique used in their study, providing a foundation for understanding the detailed data analysis that will follow.

Throughout the webinar, we will explore various aspects of AAVs, such as their structure, stereotypical variations, and the importance of confirming their critical quality attributes for therapeutic applications. The presenters will discuss the challenges and current techniques used in AAV characterization, including ELISA, size exclusion chromatography, and more sophisticated methods like electron microscopy and reverse-phase LC-MS. This comprehensive analysis not only highlights the advancements in AAV research but also equips participants with the knowledge to understand the intricate details of protein analysis and the capabilities of LC-MS in modern virology research.