EAD offers a unique fragmentation mechanism that preserves post-translational modifications and provides high-quality fragmentation data, including for disulfide-bonded peptides and differentiation between amino acid isomers. This enhances the characterization of complex biotherapeutics. The seamless integration between the ZenoTOF 7600 and Protein Metrics’ Byos platform demonstrates how advanced instrumentation coupled with sophisticated software solutions can significantly improve the analysis and quantification of biotherapeutics, including ADCs.