Advancements in Multi-Protein Quantitation for Host Cell Protein Discovery
Discover new multi-protein quantitation workflows designed for biopharmaceutical discovery. This tool enables rapid, automated analysis of thousands of proteins, from early cell line development to drug purity verification. Leverage Byosphere® cloud platform’s advanced dashboards to compare data across labs, projects, and regions in a GxP-ready environment. Perfect for real-time monitoring of host cell proteins and more!
This poster presents a novel workflow comparing LC-MS and icIEF charge variant analysis for biotherapeutics. The system integrates fractionated samples from Maurice systems with Byos® software, enabling the superimposition of icIEF and mass spectrometry data. This approach simplifies charge variant characterization and facilitates direct comparison of charge profiles, providing deeper insights into protein composition and modification patterns.
Automated Spectrum Annotation and Structure Disambiguation of Released N-linked Glycans
Glycosylation is a crucial yet complex protein modification. This poster presents an all-MS approach for analyzing glycan structures, simplifying the study for non-specialist labs. By using MS1 for monosaccharide composition and MS2 for topology, it enables precise glycan analysis. With Byos®, users can map glycan structures, perform fragment matching, and explore glycan fine structures through tandem MS, aiding in more confident and automated glycan structure determination.
Digested Oligonucleotide IP-RPLC-MS/MS Characterization of mRNA Sequence, 5’ Cap, and 3’ PolyATail
This poster presents a comprehensive workflow for characterizing mRNA PQAs through digested oligonucleotide sequence mapping. Using LC-MS/MS, it identifies the 5’ cap, oligo sequence, and 3’ PolyA tail in a single assay. Byos® software automates the analysis, providing reliable isomer assignment, mass matching, and sequence coverage, ensuring the safety and efficacy of mRNA therapeutics.
A Middle-Level Approach to Simplify Sample Preparation and Data Analysis for
Characterization of the Fusion Protein Blinatumomab
This poster showcases a middle-level analysis of the fusion protein Blinatumomab using GlySERIAS protease. By digesting glycine-rich linkers and analyzing fragments with LC-MS/MS, the workflow in Byos® automatically deconvolutes and identifies fragment masses, confirming cleavage sites. This method provides efficient analysis of complex biopharmaceuticals by targeting resistant linker regions and utilizing automated data tools for accurate sequencing.
