Improve MS Quality of Intact Proteins in Complex Mixtures
In the video, Steve Broom presents the Byos platform for protein analysis, highlighting intact mass spectrometry and innovative methods for improved data quality.
Webinar Summary
Generating intact protein charge envelopes in mass spectrometry requires careful sample prep, often excluding reagents commonly used to prevent non-specific analyte interactions with vessels. Examples include salts (NaCl, KCl, MgCl2) and detergents (NP-40, SDS, Tween-20), which can suppress signals or complicate spectra.
MOBILion Systems developed a high-resolution ion-mobility system that separates compounds by collisional cross section (CCS) post-ionization, aiding intact protein analysis. This seminar will discuss how combining ion-mobility with Protein Metrics HRIM software enhances data processing and mass spectral accuracy.
More Resources
GUIDEQuick Start Guide to Intact Analysis
An easy guide to getting started with intact, reduced, ADC, intact reconstruction, and IntaBio icIEF-MS workflows.
VIDEO SERIESWorking with Intact Mass Workflows
Run intact mass workflows in Byos. Manage data, sequences, masses, deconvolution, and generate reports for ADCs, glycosylation, and oligos.
VIDEO SERIESResearch Spotlight on Intact Mass
Watch this video on a novel serum stability assay, enhancing accuracy in biologics screening with in vitro methods.
PUBLICATIONIntact Reconstruction for Bi-Specific Antibody Characterization
Integrating tryptic digest peptide data with intact MS data using the new Reconstruction Workflow.