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Featured Resources

Unraveling the Twin Tales of Biosimilar and Innovator Glycans

N-glycans are omnipresent in the Fc region of monoclonal antibody products. Modifications to a glycan profile can impact a protein’s stability/folding, binding affinities, effector functions, clearance rates, and/or immunogenicity.

Automated de novo mAb Sequencing for a Modern Biotech

Using the right practical tools, de novo sequencing now can be easily turned into an in-house, higher throughput procedure with extremely high confidence.

PTM Analysis – How to Marry Speed and Confidence in R&D

John Reed covers the ins and outs of PTM analysis. He touches on peptide identification and the use of standard curves and weighted averaging of XICs - and a little bit of ice cream.

Analysing the SARS-CoV-2 glycan shield with mass spec

Why are we interested in virus glycosylation in general? Max Crispin from the University of southampton gives us an intrudction to the topic as well as a deep dive into the elucidation of the SARS-CoV-2 Glycan shield

Monitoring System Suitability – Byosphere Dashboards

Comparing batches? Assessing digestion completion? Tracking over-alkylation? Learn about the rational for the types of dashboard constructed to monitor these important aspects of laboratory operations.

EU UGM 2022 – EPO analysis using ZIP Chip CE-MS and Byos Software

Marshall Bern details the usage of Intact Mass deconvolution in order to tease apart the complex glycosylation profiles of EPO

EU UGM 2022 – LC-MS and LC-MS/MS Analyses of Adeno-Associated Viruses Anc80

Jerome Jonveaux describes peptide and intact workflows developed for quantification of PTMs and comparison of AAV samples

7:53

Proteomic Analysis of Adenovirus 5 by UHPLC-MS/MS: Development of a Robust and Reproducible Sample Preparation Workflow

The novel workflow for AdV VP analysis affords substantially lower analysis times than traditional peptide mapping methods, which require the use of several proteases and long sample processing for sufficient in-depth characterization.

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Application Notes

Accelerating Multi-Attribute Methods (MAM) – Automating Workflows from Sample Prep to Data Analysis with Vendor-Neutral Software
Multi-Attribute Monitoring (MAM) in Byos® Part A – CQA Identification (和訳版)
Multi-Attribute Monitoring (MAM) in Byos® Part B – New Peak Detection & CQA Quantification (和訳版)
Validation of Fully Automated Sample Prep Protocol for Peptide Multiple-Attribute Monitoring (MAM) using Quantitative Peptide Analysis
Byos Disulfide Bond Analysis (和訳版)
In-silico Disulfides CSV (和訳版)
How Janssen, Genentech, and Amgen Create a Highly Automated Analysis of Antibody Chain Shuffling in Bispecific Antibodies
Rapid Identification and Quantitation of Disulfide Bonds in Infliximab – Remicade vs Remsima
How to Analyze Biosimilars with Confidence (中译版)
Byos® Sequence Variant Analysis (SVA) (中译版)
Automating Sequence Variant and Post-Translational Modification Analysis (中译版)
Informative Protein Charge Variant Analysis by Native Spray Mass Spectrometry
Detached Glycan Analysis in Byos®
Automating Data Processing for Glycan and Peptide Mapping: Leveraging Byos® to Improve Efficiency and Quality
Glyco-Analysis: Byonic™: N-Linked Glycopeptide Analysis (和訳版)
Glyco-Analysis: Byonic™: O-Linked Glycopeptide Analysis (和訳版)
Specific and Confident Glycation Site Mapping in Therapeutic Proteins Using Automated Data Processing
Byonic™: Modification Fine Control™
Byonic™: Wildcard Search™
Byonic™: Mass Tolerance Settings
Crosslink Searches in Byonic
Byonic™: Focused Protein Databases
Integrated software platform for analyzing hydrogen-deuterium exchange data for solvent accessibility of the Marburg filoviral protein (2021)
HCP Analysis: Host Cell Protein (HCP) Analysis in Byos®
Drug-to-antibody ratio quantification using native SECMS analysis of antibody–drug conjugates
Evaluation of a New Software Tool for Assisting with the siRNA Metabolite Identification by LC/MS
Asparagine Deamidation Quantified by Byos® (和訳版)
Automated Data Filtering for Rapid Sample Comparison of Human milk Glycoproteins
Characterization of Adeno-Associated Virus Capsid Proteins Using Hydrophilic Interaction Chromatography Coupled with Mass Spectrometry Using Byos®
Chromatogram labeling with CSVs
Intact and Chromatography Trace Peak Settings
AAV Characterization: Direct Bottom-up versus Intact MS analysis Comparison (中译版)
Intact Profiling and Visualization of Urinary Prostate-Specific Antigen (PSA) Profiles from Pooled and Individual Patient Samples
Coagulation factor IX analysis in bioreactor cell culture supernatant
Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins
Highly Automated Analysis of Chain Shuffling in Bispecific Antibodies
Rapid Protein Characterization by the ZipChip-MS Platform
How to Maintain Data Quality across Time and Mass Spectrometry Platforms
Comparability and Biosimilarity Assessment with Protein Metrics
Released Glycan Analysis in Byos® and Using Cartoons
Byosphere™ Enterprise Platform: Bringing Speed and Depth to Protein Mass Spec Analysis
Byosphere® Enterprise Platform: Manage LC-UV-MS Data Seamlessly with Byosphere®
Intact Reconstruction of a Lysine Conjugated ADC from Quantitative Peptide Mapping Data, Generated Using an Arginine-specific Protease
Sciex – Characterization of a multi-specific antibody therapeutic with peptide mapping
Optimization of a Label-Free Peptide Quantitation Strategy for Analysis of Therapeutic Proteins: Insights from Roche’s Assessment and Byos Implementation
Intact Mass: Workflow Setup for Low MW (<10 kDa) Isotopically Resolved Peptides
Intact Analysis of a Purified Therapeutic Protein Incorporating Top-Down Fragmentation

tutorials

Byosphere: Introduction
6:23
Byosphere: Getting Started
13:26
Byosphere: Custom Metadata Fields
0:50
Byosphere: Dashboards
15:45
Byosphere: Advanced Queries
4:30
Byosphere: Intact Web Analysis
15:06
Byosphere: GxP Compliance
3:51
Byosphere: Audit Trails
1:23
Byosphere: Auto Scaling
1:59
Byonic: Byonic Introduction
7:11
Byonic: Preview Overview
6:11
Byonic: Modification Fine Control
7:45
Byonic: Advanced Tab
5:38
Intact Mass: Workflows Overview
3:33
Intact Mass: Adding data and peak integration options
2:47
Intact Mass: Adding sequences
4:51
Intact Mass: Delta Mass
3:55
Intact Mass: Deconvolution settings
3:19
Intact Mass: GUI
3:41
Intact Mass: Using .csv workflows
2:36
Intact Mass: Reports Overview
6:35
Intact Mass: ADC Workflow
3:01
Intact: Advanced Commands
3:58
Intact Mass: Oligonucleotides
11:49
Peptide: Workflows Overview
2:00
Peptide: Creating Projects, Samples and Proteins Tabs
2:13
Peptide: Processing Nodes: Byonic Intro
1:37
Peptide: Processing Nodes: Byonic Details
7:48
Peptide: Byonic Results
3:07
Peptide: Processing Nodes: Byologic Options
9:03
Peptide: GUI, Overview, Validation, Comments, Labels
7:57
Peptide: Deamidation
4:20
Peptide: Report Overview
6:56
Peptide: Peptide Manager Explained
10:28
Peptide: Glycan Cartoon
11:06
Peptide: Advanced Commands
3:58
Peptide: IsoAsp
4:15
Peptide: disulfides
12:02
Chromatogram: Workflows Overview
1:57
Chromatogram: Integration Set Up
6:02
Chromatogram: Creating a MS2 Reference Project
3:08
Chromatogram: Creating an in-silico Reference Project
5:01
Chromatogram: Creating a Comparison Project
2:30
Chromatogram: Baseline Anchors
2:42
Chromatogram: Released Glycans Introduction
1:08
Chromatogram: Released Glycans Project Creation
5:55
Chromatogram: GUI & Project Interface
6:24
Byos Reporting Basics
14:06
Disulfide Bonding in Byos
4:24
Ligand Binding with High Res Native MS
4:24
Data Visualization for HCPs
5:55
Unanticipated N- and O- Glycopeptides
3:31
Byos Multi Step Searches
0:56
HDX: Introduction
6:51
HDX: Reports
7:46
HDX: Inspection View
6:22
Byonic: Byonic Only workflow in Byos
4:40
Byonic: Preview in Byosphere
1:35
Reconstruction: Intact Reconstruction
8:02
Chromatogram: Processing Biophysical (non-MS) data
5:55
Digested Oligos: Introduction
2:06
Digested Oligos: Workflow Set Up
9:46
Digested Oligos: Modifications
6:55
Digested Oligos: Investigation View
6:21
Digested Oligos: Customizing Layout
4:55
Digested Oligos: Reporting
2:54
Digested Oligos: Table View
14:12
Reconstruction: Charge Variant Reconstruction
5:01

webinars

アジレント、プロテインメトリックスが提供する医薬品オリゴ核酸分析の課題と解決法
59:10
Reconstructing cIEF Profiles Using Peptide Mapping Data
35:05
new Automating Antibody Characterization with Mobile Affinity Sorbent Chromatography
52:42
new Enhancing Polyclonal IgG Product Characterization with Byosphere
46:30
new Improving Mass Spectral Quality of Intact Proteins in Complex Mixtures
52:57
new Comparative analysis of MS/MS search algorithms in label-free shotgun proteomics for monitoring host-cell proteins
42:40
効率的なMAM解析
50:00
NA_UGM24: Dashboards and Deep Query
8:01
Bovine milk immunoglobulin G: NeuAc to NeuGc
39:03
NA_UGM24: State of the Union
29:10
NA_UGM24: Navigating the Future of Laboratory Automation
29:10
NA_UGM24: Intact Mass Characterization of Multispecific Therapeutic Antibodies
29:01
NA_UGM24: How Protein Metrics Saved Me From Carpel Tunnel
29:54
NA_UGM24: A 3 body problem: Automation, Integration & Human
19:58
NA_UGM24: Automating QC In Discovery Therapeutics : Leveraging Byosphere to Maximize Potential in High Throughput Intact MS QC
35:01
NA_UGM24: Robust automated quantitation with improved feature detection, peak bounding, and cross-sample feature association
17:09
NA_UGM24: MS analysis of intact oligonucleotides
29:37
NA_UGM24: Panel Discussion – Gearing Up For Smarter Workflows
46:27
Unraveling the Twin Tales of Biosimilar and Innovator Glycans
41.26
Increasing Analytical Efficiency at a CDMO
18:38
From Here to Byosphere
42:43
HDX-MS analysis of SARS-CoV-2 spike ectodomain
32:54
Spotting the Outliers and New Peaks in MAM Runs Automatically
46:12
JUGM 2023: 生体試料中のADCのDAR推移の分析におけるBYOSの活用例
24:06
JUGM 2023: 製品ロードマップ
15:17
JUGM 2023: 目からうろこByos/Byonicの成り立ちから有効活用のtipsまで
28:00
JUGM 2023: タンパク質特性解析のデジタル化
14:54
JUGM 2023: ByosがMulti-Attribute Method(MAM)に貢献できること
17:19
JUGM 2023: Byosphereご紹介
18:33
JUGM 2023: ヒスチジン水素重水素交換質量分析法による生体内タンパク質・リガンドの網羅的な結合評価への挑戦
18:18
Resolving Antibody Aggregation During Discovery & Development
27:29
EU UGM 2023: Impurity Characterisation of modified siRNA using Byos Oligo Workflow
27:36
EU UGM 2023: Enhancing the Dimensionality of Glycoproteomics
33:27
EU UGM 2023: Protein Metrics’ MAM application as a robust standard for PTM detection in large scale forced degradation studies
23:18
EU UGM 2023: The Growing Importance and Changing Needs for Data Analysis & Informatics – US UGM
26:24
EU UGM 2023: Annotation of topdown mass spectra of proteins and oligonucleotides
37:52
EU UGM 2023: Bispecific Antibody Formats – Challenges in Peptide Mapping and how a Multi Enzyme Approach improves the Analysis of Sequence Variants
38:49
EU UGM 2023: Byosphere assisted biopharmaceutical development
19:15
EU UGM 2023: Friends with benefits: Glycoproteomics meets Chemical Biology
20:31
EU UGM 2023: Introduction & State of the Union
25:53
Neutrophil azurophilic granule glycoproteins – atypical glycans
54:23
Mass Spectrometry Techniques Enabling Accelerated Process and Product Development of Bispecific Antibodies (中译版)
33:28
WCBP 2023: Unearth the value of your complex biopharma data
14:07
Characterizing the glycan shield of the Hepatitis C E1E2 glycoprotein complex using mass spectrometry
33:32
Tackling the Glycan Isomer Challenge for your Biotherapeutics
38:23
質量分析によるオリゴヌクレオチド分析：配列の表示および自動化はもはやシーシュポスの作業（果てしない徒労）ではない
56:21
High throughput screening and data analysis using Native Mass Spectrometry and Byos
34:46
Monitoring System Suitability – Byosphere Dashboards (中译版)
33:49
PEGS 2022 – Mining Biological Attributes with Dashboards
30:00
2022 Bioprocessing Summit: Autonomous Pipeline for Characterizing Biotherapeutics
28:53
EU UGM 2022 – Importance of Data Analysis & Informatics in Development – Dashboards Latest
36:55
EU UGM 2022 – Introduction & State of the Union
30:46
EU UGM 2022 – Development of a HILIC method for detection of low abundant N-glyco-peptides
30:46
EU UGM 2022 – Structural Determinants Regulating Viral Spike Glycosylation and Biomolecular Interactions
37:22
EU UGM 2022 – Rapid Analysis of Oligonucleotides by High-Resolution Accurate Mass CE-MS
31:49
EU UGM 2022 – LC-MS and LC-MS/MS Analyses of Adeno-Associated Viruses Anc80
18:56
EU UGM 2022 – EPO analysis using ZIP Chip CE-MS and Byos Software
34:14
EU UGM 2022 – Addressing MS Data Bottlenecks and Biopharmaceutical Case Studies
43:02
Stanford SUMS Seminar – Connecting LC-MS data analysis with non-MS techniques
48:47
Intact Profiling and Visualization of Urinary Prostate-Specific Antigen Profiles
37:51
Generating Wisdom from Raw Data – The Post Genomic World
17:26
The Sweet Science of Sparkling Wine
18:23
Assigning function to the structure of HIV and SARS-CoV-2 glycan shields
25:08
Analytical Characterization of Oligonucleotides by LC-HRMS and BYOS
47:05
Three Channel Charge Deconvolution
18:47
Byosphere™ Enterprise Platform – Metadata & Dashboards
19:13
Harnessing Novel MS Approaches (MAM, IEX-MS) to Accelerate Biotherapeutic Development and Analysis
43:00
Bringing your analysis to the enterprise: Introducing Byosphere™
30:56
Ensuring Safety and Efficacy of Biotherapeutics Through In-Depth LC-MS Sequence Variant Analysis
45:57
EAD: Making characterization of complex biotherapeutics routine
38:20
EPO: Separation and Analysis
54:45
Oligo Analysis Simplified: Get Your Answers Faster with Microchip CE-MS
51:08
Bispecific MS Analysis and Cell Line Development
57:17
Recent Progress in MS-Based Workflows and Case Studies from a Biopharmaceutical Development Lab
52:09
Comprehensive Adeno-Associated Virus CQA Analysis with LC/MS
59:20
Biosimilar Characterization
29:19
Characterizing Next Generation Biotherapeutics with Novel Ion Trapping and Alternative Fragmentation
36:29
Implementing an Enterprise Solution for MAM with LC-UV-MS
45:51
Data Visualisation for Routine Host Cell Protein Analysis
37:56
Oligonucleotide Characterization Using the BYOS Intact Mass Workflow
17:42
Real Time Monitoring of Bioprocess & Beyond – A Process Analytical Perspective
1:01:35
Automated de novo mAb Sequencing for a Modern Biotech
42:39
All Tangled Up: High Resolution Neuroproteomics of the human p-Tau Interactome
34:06
Keeping it all together – Bacterial Cell Envelope Structure
30:17
Automated Feedback Control of Protein Characteristics in a Perfusion Bioprocess
20:15
Automating data collecting and analysis in the X-ray hydroxyl radical footprinting method
19:34
Rapid Intact Charge Variant Analysis with Coupled icIEF-MS
53:39
Analyzing the SARS Cov-2 Glycan Shield with Mass Spec
54:22
PTM Analysis – How to Marry Speed and Confidence in R&D
1:00:51
How to Advance Your Host Cell Protein Analysis with Mass Spectrometry
56:09
An Implementation of Vendor-Neutral Multi-Attribute Method (MAM)
56:32
Native MS and Cell Line Development
24:47
Driving Efficiency in Pre-Clinical Development with Automated Mass-Spectrometry Analysis
48:58
Zeta 2018: Why don’t we monitor drug product profile directly?
29:04
Improving Intact Antibody and Plasma Glycoprotein Analysis with Native MS and Parsimonious Charge Deconvolution
56:09
Streamlining PTM Workflows: From Sample Prep to Data Analysis
55:34
HT Analysis of Bispecific Antibody Moieties and Glycosylation
51:04
Model Based Algorithms for Intact Mass Analysis of Biotherapeutics
21:29
Large-scale Glycopeptide Identification by Database Search
19:59

posters

ASMS 2024: Charge Variant Mass spectrometry for Analysis of biotherapeutics with Orthogonal and Fractionated sampling
ASMS 2024: Utilization of Intact Reconstruction to Characterize Bi-specific Antibodies (Video 3:03)
ASMS 2024: Assessment of different MS Label-Free Quantitation Strategies for Therapeutic Proteins
ASMS 2024: Network method for building a sample-specific glycan database for N-linked glycosylation from MS/MS data (中译版)
ASMS 2024: Determination of drug-to-antibody ratio of antibody-drug conjugate in biological samples using microflow-liquid chromatography/HRMS (中译版)
ASMS 2024: Optimization and Characterization of Disulfide Linkages in the Complex Cysteine Engineered Stapled scFv for Bispecific Antibodies (中译版)
ASMS 2024: Computer Modeling of the Effectiveness of MS1 and MS2 for mRNA Sequence Confirmation (中译版)
ASMS 2024: A Middle-Level Approach to Simplify Sample Preparation and Data Analysis for Characterization of the Fusion Protein Blinatumomab (中译版)
CASSS 2023: Optimization of a Liquid Chromatography-Tandem Mass Spectrometry mRNA Sequence Mapping Workflow (中译版)
Fragmentation-free characterization of glycopeptides with ion mobility
MSSJ 2023 – High-Sensitivity Charge Deconvolution Algorithm for Low-Abundance Biological Macromolecules
ASMS 2023: Orthogonal techniques for LCMS characterization of a lysine-conjugated ADC (和訳版) (中译版)
ASMS 2023: Histidine hydrogen-deuterium exchange (His-HDX) mass spectrometry for identifying protein-ligand interactions
ASMS 2023: Sample to Result Workflow for the Investigation of Biosimilars vs Innovator Cetuximab by Charge Variant Analysis using Microchip CE-MS
ASMS 2023: Automated Spectrum Annotation and Structure Disambiguation of Released N-linked Glycans
ASMS 2023: Coupling online buffer exchange with automated data processing for screening designed protein complexes by native mass spectrometry
ASMS 2023: A Real-time Informatics Pipeline Enables Screening of Biologics during High-Throughput Expression (中译版) (Video Poster 5:10)
ASMS 2023: Investigations of a Bispecific Antibody Dimerization via Hydroxyl Radical Footprinting
ASMS 2023: Effects of Fab glycosylation on anti – SARS – CoV – 2 antibody – antigen binding assessed by SEC and LC – MS
TIDES 2022 – A Microchip CE-MS Based End-to-End Solution for the Analysis of Synthetic Oligonucleotides
ASMS 2022 – High-throughput Monitoring of Biotherapeutic Critical Quality Attributes with High-Resolution Ion Mobility-Mass Spectrometry (HRIM-MS)
ASMS 2022 – High Throughput Automation Platform of Intact Mass Spectrometry Data to Support Cell Line Development (CLD) of Multi-specific Antibodies (和訳版)
ASMS 2022 – Tandem Mass Spectrometry Metrics for Reliable and Automated Assignment of Protonated Glycan Isomers
ASMS 2022 – Comprehensive site-specific glycan profiling of a protein-based flu vaccine using electron activated dissociation (EAD) (和訳版)
ASMS 2022 – Comprehensive characterization of complex linkage structures in a bispecific monoclonal antibody (mAb) using electron activated dissociation (EAD) (和訳版)
ASMS 2022 – Characterization of Isomeric O-Glycopeptides using µLC, Differential Mobility Spectrometry, Collision Induced and Electron Capture Dissociation
ASMS 2022 – Chromatography ‘super-resolution’: Improved HDX mapping using long gradients and time warping (和訳版)
ASMS 2022 – Native Mass Spectrometry and Fast Photochemical Oxidation of Proteins (FPOP) for Characterization of TREM2 interaction with Amyloid β1-42 peptide
ASMS 2022 – High-Sensitivity Charge Deconvolution Algorithm for Low-Abundance Biological Macromolecules (和訳版) (中文视频)
ASMS 2022 – LC-MS/MS proteomics reveals anticancer compounds from Lansium domesticum against NSCLC cells target mitochondrial processes
ASMS 2022 – Survival of infant formula N glycoproteins across simulated infant gastrointestinal digestion
ASMS 2022 – Epitope Mapping of HER2 with Single Amino Acid-Level Resolution using a Combination of Plasma-Initiated Radical Trifluoromethylation and Hydroxyl Radical Footprinting
ASMS 2022 – Capillary Electrophoresis Mass Spectrometry (CEMS) of Oligonucleotides using a 908Devices ZipChip
ASMS 2022 – Characterization of Biotherapeutics with High-Resolution Ion Mobility-Mass Spectrometry
Bottom-up and Top-down Disulfide Bond Mapping of Beta-lactoglobulin on a Q-TOF with the Capability to Perform both CID and ECD
ASMS 2022 – Chromatography ‘super-resolution’: Improved HDX mapping using long gradients and time warping (Video)
BMSS 2022: Oligonucleotide Analysis by Mass Spectrometry: Sequence Display and Automation is no longer a Sisyphean Task (和訳版)
BMSS 2022: High Sensitivity Charge Deconvolution Algorithm for Low Abundance Biological Macromolecules
CASSS 2022: Continuous Monitoring of System Suitability with Byosphere® Automation and Dashboards (Video 4:57)
CASSS 2022: A Streamlined Workflow for Rapid Charge Variant Analysis of Cetuximab innovator and two biosimilars by Microchip CE-MS with Data Analysis
CASSS 2022: Oligonucleotide Analysis by Mass Spectrometry: Accurate Annotation of Fragmentation Spectra
FOB 2022: Streamlining CQA analysis of Biosimilars and transforming information sharing across the globe
FOB 2022: Enabling a Multi-Attribute Method Comparison of Biosimilars
HUPO 2022: Accelerated O-glycopeptide search with isobaric filtering (Video 1:29)
HUPO 2022: N-Glycoproteomics MS/MS Data Analysis Benefits from Glycomics-lnformed Search Strategies
ASMS 2021: Analytical Characterization of Oligonucleotides by LC HRMS
ASMS 2021: Comprehensive Adeno-Associated Virus (AAV) Critical Quality Attribute Analysis with LC-MS (和訳版)
ASMS 2021: High-Throughput Product Quality Assessment of Protein Therapeutics on ZipChip-MS platform
ASMS 2021: Novel On-Chip Nebulizer for Reliable ESI andicIEF-MS BasedCharge Variant Identificationof Biopharmaceuticals
ASMS 2021: Mapping the Epitope Interactions and Conformational Changes to HER2 upon Herceptin Binding using Plasma Induced Modification of Biomolecules (PLIMB)
ASMS 2021: RapidFire-MS (RF-MS) as an Alternative to LC-MS for Rapid Assessment of Glycoforms in Therapeutic Antibodies
ASMS 2021: The Utility of Nanoparticle Protein Coronas for Studying the Plasma Glycoproteome
ASMS 2021: Applications of electron activated dissociation for next-generation biotherapeutic characterization
ASMS 2021: Assessing and Correcting for Electrospray Ionization Response of mAb Fragments Post Mass Deconvolution
ASMS 2021: High Throughput Biotherapeutic Glycosylation Profiling Using High Resolution Ion Mobility MS
ASMS 2021: Real Time Antibody Digestion by Rapid Flow Injection Mass Spectrometry
ASMS 2021: Kitted universal MAM: Automatable Sample Processing for all Stages of Biological Drugs
ASMS 2021: Multi-attribute Monitoring of Monoclonal Antibodies by icIEF-MS
Oligonucleotide Analysis by Mass Spectrometry Sequence Display and Automation is no longer a Sisyphean Task (Video)
Analytical Characterization of Oligonucleotides by Liquid Chromatography – (LC) High Resolution Mass Spectrometry (HRMS)
Oligonucleotide Analysis by Mass Spectrometry: Sequence Display and Automation is no longer a Sisyphean Task (和訳版)
Integrated software platform for analyzing hydrogen-deuterium exchange and oxidative footprinting data for solvent accessibility
Antibody De Novo Sequencing by LC MS/MS using Highly Automated Sample Preparation and Data Processing (Video)
ASMS2020: Use of PASEF for Accelerated Protein Sequence Confirmation and de novo Sequencing with High Data Quality
A Glycan Wildcard Search for Identifying Glycoprotemic Dark Matter (Video 3:07)
CASSS2020: Assaying Protein / Ligand Binding with High-Resolution Native Mass Spectrometry
CASSS2020: Wildcard™ and Diagnostic Ion Filtering to Find Unanticipated N- and O-linked Glycopeptides
ASMS2020: Assaying Protein / Ligand Binding with High-Resolution Native Mass Spectrometry
ASMS2020: Systematic examination of protein/glycopeptide extraction methods and MS/MS fragmentation techniques for milk glycoproteins survival across infant digestion
ASMS2020: MS/MS Filtering and Wildcard™ Searches for Novel N-and O-linked GlycopeptideIdentifications
ASMS2020: Reconstructing Protein Charge Heterogeneity from a Bottom-Up Approach
ASMS2020: Unravelling Hundreds of Proteoforms of Industrial Enzymes
ASMS2020: MS Characterization of Extensin Peptides from Cell Walls: Identification of Intra- and Intermolecular Cross-links and Abundance of Internal Fragment Ions
ASMS2020: Making LC-MS Sensibly Useful for Host Cell Protein Applications (和訳版)
ASMS2020: Evaluation of a New Software Tool For Assisting with the siRNA Metabolite Identification by LC-MS
ASMS2020: Ion Mobility Mass Spectrometry of Glyco- and Phospho-Peptides
ASMS2020: Cyclic Peptide Tandem-MS Search Software
Superposition of Orthogonal Methods – Intact Reconstruction from Peptide Mapping
ASMS2019: Charge Deconvolution and Automatic Sequence Matching for Oligonucleotides
HOS2019: Integrated Software Platform for Analyzing Hydrogen-Deuterium Exchange and Oxidative Footprinting Data Ebola
Glycobiology2018: Intact & Native Mass Analysis of Glycoproteins
Mapping the Interactions of PKNB with Small Molecule Inhibitors Using Plasma Induced Modifications of Biomolecules (PLIMB)
Determining solvent access of HNE adducted myoglobin with hydroxyl radicals generated from PLIMB
NIIMBL2019: Rapid Assessment of CQAs Using Imaged CIEF-MS for Real-Time Bioprocess Decision Making
ASMS2019: Charge Deconvolution of Surface-Induced-Dissociation (SID) Spectra of Protein Complexes
ASMS2019: Cleavage of Intact Monoclonal Antibodies by Cathepsins L and D Studied by Native Mass Spectrometry
ASMS2019: Isotope Selection in Label-Free Quantification and its Effects in Biopharmaceutical Characterization
Highly Automated Analysis of Antibody Chain Shuffling in Bispecific Antibodies (和訳版)
ASMS2018: Host Cell Proteins (HCPs) in Plasma-Derived Biotherapeutics
CASSS2018: High Throughput LC-MS Analysis of Intact Light and Heavy Chain Supporting mAb Screening and Cell Line Characterization
CASSS2018: Streamlined MAM for New Peak Detection, Comprehensive Data Inspection, and Confident Reporting
CASSS2018: Automatic N-Glycan Analysis of Therapeutic Glycoproteins using Byos Vendor-Neutral Software
HUPO2018: Charge Deconvolution of Hard-to-Deconvolve Mass Spectra
WCBP2018: Host Cell Proteins (HCPs) in Plasma-Derived Biotherapeutics
WCBP2018: Multi-Attribute Comparison of Innovator and Biosimilar Filgrastim
ASMS2018: Automatic End-to-End De Novo Sequencing (including I/L) of Antibodies with EThcD Fragmentation
ASMS2018: Integrated Software Platform for Analyzing Hydrogen-Deuterium Exchange and Oxidative Footprinting Data West Nile Virus
ASMS2018: Automatic Detection of Clipped Monoclonal Antibodies from High-Resolution Native Mass Spectrometry
PEGS2018: Antibody Analysis with Native Mass Spectrometry and Parsimonious Charge Deconvolution
WCBP2018: Antibody Analysis with Native Mass Spectrometry and Parsimonious Charge Deconvolution
ASMS2017: Analytical Characterization and Comparison of Filgrastim and Its Biosimilar
WCBP2017: Multi-attribute Method Comparison of Infliximab
WCBP2017: Harmonizing Physicochemical Characteristics
ASMS2015: Rapid Identification and Quantitation of Disulfide Bonds in Infliximab (Remicadevs Remsima)
Warren Workshop 2014: Byonic™ Glycopeptide Analysis
ASMS2014: Preview–>Byonic™ Workflow
ASMS2014: Top-down Analysis with Byonic™
ASMS2013: Byonic™ Phospho & Glycopeptides
Toward chemical footprinting at residue resolution: FPOP data analysis of IL23 – antibody binding
Assaying Protein / Ligand Binding with High-Resolution Native Mass Spectrometry
Simplified and automated analysis of the highly glycosylated therapeutic protein Erythropoietin (Video Poster 4:55)
Complex glycoprotein characterization using CE-MS and Automated Analysis
Challenges and Strategies in Glycan Analysis by MAM
TIDES2024: Oligonucleotide Samples are Amenable to Automated LCMS Analysis
A Novel in vitro Serum Stability Assay for Antibody Therapeutics incorporating Internal Standards (Video 6:56)
IRT 2024: Optimization of a Liquid Chromatography-Tandem Mass Spectrometry mRNA Sequence Mapping Workflow
CASSS 2024: Charge Variant Mass Spectrometry for Analysis of Biotherapeutics with Orthogonal and Fractionated Sampling
CASSS 2024: Automated Spectrum Annotation and Structure Disambiguation of Released N-linked Glycans
CASSS 2024: A Middle-Level Approach to Simplify Sample Preparation and Data Analysis for Characterization of the Fusion Protein Blinatumomab
CASSS 2024: Advancements in Multi-Protein Quantitation for Host Cell Protein Discovery copy
CASSS 2024: Digested Oligonucleotide IP-RPLC-MS/MS Characterization of mRNA Sequence, 5’ Cap, and 3’ PolyATail

published articles

Peak Filtering, Peak Annotation, and Wildcard Search for Glycoproteomics
Vulnerabilities in coronavirus glycan shields despite extensive glycosylation
TBK1 phosphorylates mutant Huntingtin and suppresses its aggregation and toxicity in Huntington’s disease models
Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins
Site-specific analysis of the SARS-CoV-2 glycan shield
Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)
Optimal Dissociation Methods Differ for N- and O-Glycopeptides
Automated Antibody De Novo Sequencing and Its Utility in Biopharmaceutical Discovery
Localization of Protein Complex Bound Ligands by Surface-Induced Dissociation High-Resolution Mass Spectrometry
Development of an LC-MS/MS peptide mapping protocol for the NISTmAb
Identification of Sialic Acid Linkages on Intact Glycopeptides via Different Chemical Modification Using IntactGIG-HILIC
Parsimonious Charge Deconvolution for Native Mass Spectrometry
Biosimilarity under stress: A forced degradation study of Remicade® and Remsima™
A Multidimensional Analytical Comparison of Remicade and the Biosimilar Remsima
Extended O-GlcNAc on HLA Class-I-Bound Peptides
Characterization of Drug-Product-Related Impurities and Variants of a Therapeutic Monoclonal Antibody by Higher Energy C-Trap Dissociation Mass Spectrometry
Polylactosaminoglycan glycomics: enhancing the detection of high molecular weight N-glycans in MALDI-TOF profiles by matched filtering
Glycoproteomic analysis of the secretome of human endothelial cells
Byonic™ advanced peptide and protein identification software
Constrained de novo sequencing of conotoxins
Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis
Proteomic changes in cerebrospinal fluid of presymptomatic and affected persons carrying familial Alzheimer disease mutations
Preview: A Program for Surveying Shotgun Proteomics Tandem Mass Spectrometry Data
Recent developments in quantitative proteomics
Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools
Comment on “Unbiased statistical analysis for multi-stage proteomic search strategies”
Two-dimensional target decoy strategy for shotgun proteomics
Conversion of methionine into homocysteic acid in heavily oxidized proteomics samples
Elucidating in vivo structural dynamics in integral membrane protein by hydroxyl radical footprinting
Glycan family analysis for deducing N-glycan topology from single MS
Automated N-glycopeptide identification using a combination of single- and tandem-MS
Lookup peaks: a hybrid of de novo sequencing and database search for protein identification by tandem mass spectrometry
An embeddable molecular code for Lewis X modification through interaction with fucosyltransferase 9
Ultraviolet Photodissociation and Activated Electron Photodetachment Mass Spectrometry for Top-Down Sequencing of Modified Oligoribonucleotides
Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis
Attribute Analytics Performance Metrics from the MAM Consortium Interlaboratory Study
Streamlining the Characterization of Disulfide Bond Shuffling and Protein Degradation in IgG1 Biopharmaceuticals Under Native and Stressed Conditions
Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry
An Integrated Proteomic and Glycoproteomic Investigation Reveals Alterations in the N-Glycoproteomic Network Induced by 2-Deoxy-D-Glucose in Colorectal Cancer Cells
Structure of the hepatitis C virus E1E2 glycoprotein complex
Microflow Liquid Chromatography Coupled to Mass Spectrometry (μLC–MS) Workflow for O-Glycopeptides Isomers Analysis Combining Differential Mobility Spectrometry and Collision Induced and Electron Capture Dissociation
Extending Proteome Coverage by Combining MS/MS Methods and a Modified Bioinformatics Platform Adapted for Database Searching of Positive and Negative Polarity 193 nm Ultraviolet Photodissociation Mass Spectra
Development of Software Workflow for the Rapid Detection of Cross-Linked Dipeptides
Physicochemical and biological impact of metal-catalyzed oxidation of IgG1 monoclonal antibodies and antibody-drug conjugates via reactive oxygen species
High-Sensitivity and High-Resolution Therapeutic Antibody Charge Variant and Impurity Characterization by Microfluidic Native Capillary Electrophoresis-Mass Spectrometry
Residue-Specific Impact of EDTA and Methionine on Protein Oxidation in Biotherapeutics Formulations Using an Integrated Biotherapeutics Drug Product Development Workflow
Sample Preparation Matters for Peptide Mapping to Evaluate Deamidation of Adeno-Associated Virus Capsid Proteins Using Liquid Chromatography-Tandem Mass Spectrometry
Optimized Multi-Attribute Method Workflow Addressing Missed Cleavages and Chromatographic Tailing Carry-Over of Hydrophobic Peptides
A competitive binding-mass spectrometry strategy for high-throughput evaluation of potential critical quality attributes of therapeutic monoclonal antibodies
Rapid Characterization of Antibodies via Automated Flow Injection Coupled with Online Microdroplet Reactions and Native-pH Mass Spectrometry
Diversity Matters: Optimal Collision Energies for Tandem Mass Spectrometric Analysis of a Large Set of N-Glycopeptides
Physicochemical characterization and functionality comparison of Humira® (adalimumab), Remicade® (infliximab) and Simponi Aria® (golimumab)
Using New Peak Detection to Solve Sequence Variants Analysis Challenges in Bioprocess Development
Higher-Order Structure Characterization of NKG2A/CD94 Protein Complex and Anti-NKG2A Antibody Binding Epitopes by Mass Spectrometry-Based Protein Footprinting Strategies
Detailed Analytical Characterization of a Bispecific IgG1 CrossMab Antibody of the Knob-into-Hole Format Applying Various Stress Conditions Revealed Pronounced Stability
“Lab of the Future”-Today: Fully Automated System for High- Throughput Mass Spectrometry Analysis of Biotherapeutics
A Highly Efficient Workflow for Detecting and Identifying Sequence Variants in Therapeutic Proteins with a High Resolution LC-MS/MS Method
Label-free shotgun proteomics: Exploiting a reliable and sensitive method to monitor residual host-cell proteins in monoclonal antibody products
Identification of Hetero-aggregates in Antibody Co-formulations by Multi-dimensional Liquid Chromatography Coupled to Mass Spectrometry
Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth PTPRZ glycosylation regulates glioma growth
Investigation of high molecular weight size variant formation in antibody-drug conjugates: microbial transglutaminase-mediated crosslinking
Impact of Trisulfide on the Structure and Function of Different Antibody Constructs
Determination of drug-to-antibody ratio of antibody-drug conjugate in biological samples using microflow-liquid chromatography-high-resolution mass spectrometry
Discovery and Characterization of an Acid-Labile Serine-Lysine Cross-Link in Antibody High-Molecular-Weight Species Using a Multipronged Mass Spectrometry Approach
Agilent – Host Cell Protein Analysis Using Agilent AssayMAP Bravo and 6545XT AdvanceBio LC/Q-TOF
Atypical Asparagine Deamidation of NW Motif Significantly Attenuates the Biological Activities of an Antibody Drug Conjugate
Site-Specific Structural Changes in Long-Term-Stressed Monoclonal Antibody Revealed with DEPC Covalent-Labeling and Quantitative Mass Spectrometry
Differential T cell immune responses to deamidated adeno-associated virus vector
Site Specific glycan analysis of the SARS-Cov2 Virus spike
Fully automated peptide mapping multi-attribute method by liquid chromatography–mass spectrometry with robotic liquid handling system
Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells
Hydroxyl radical mediated damage of proteins in low oxygen solution investigated using X-ray footprinting mass spectrometry
Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag)
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme
Identification and quantification of chain-pairing variants or mispaired species of asymmetric monovalent bispecific IgG1 monoclonal antibody format using reverse-phase polyphenyl chromatography coupled electrospray ionization mass spectrometry
Enrichment strategy and initial characterization of heterodimers enriched from a co-formulated cocktail of therapeutic antibodies against SARSCOV-2
Intact quantitation of cysteine-conjugated antibody-drug conjugates using native mass spectrometry
Insight into the Degradation Pathways of an AAV9
new Comparative analysis of MS/MS search algorithms in label-free shotgun proteomics for monitoring host-cell proteins using trapped ion mobility and ddaPASEF
new Understanding the impacts of dual methionine oxidations in complementarity-determining regions on the structure of monoclonal antibodies
new Development of a Flow Through-Based Limited Digestion Approach for High-Throughput and High-Sequence Coverage Mapping of Therapeutic mRNAs

Briefs

Orthogonal Comparison of Analytical Methods by Theoretical Reconstruction from Bottom-up Assay Data
3:03
Dragonfly Therapeutics – De Novo Sequencing
6:39
Immuto Scientific – PLIMB – Plasma Induced Modification of Biomolecules
4:45
Unravelling the glycosylation of the SARS-CoV-2 spike protein
7:02
Unexpected Modifications in Cell-Line Characterization
7:23
Automated Data Filtering for Rapid Sample Comparison of Human Milk Glycoproteins
7:01
Specific and Confident Glycation Site Mapping in Therapeutic Proteins Using Automated Data Processing
3:00
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme
5:34
Rapid Detection of Cross-Linked Dipeptides
2:38
Chemical probing provides insight into the native assembly state of a bacterial microcompartment
4:20
Streamlining the Characterization of Disulfide Bond Shuffling and Protein Degradation in IgG1 Biopharmaceuticals Under Native and Stressed Conditions
5:04
Sample Preparation Matters for Peptide Mapping to Evaluate Deamidation of Adeno-Associated Virus Capsid Proteins Using Liquid Chromatography-Tandem Mass Spectrometry (中译版)
3:31
Rapid Detection of Cross-Linked Dipeptides
2:38
Proteomic Analysis of Adenovirus 5 by UHPLC-MS/MS: Development of a Robust and Reproducible Sample Preparation Workflow
7:53
Detailed Analytical Characterization of a Bispecific IgG1 CrossMab Antibody of the Knob-into-Hole Format Applying Various Stress Conditions Revealed Pronounced Stability
5:37
BEBPA 2023 Host Cell Protein Conference – In Review
18:17
Automated Characterization of Biotherapeutics via Hardware, Software and Chemistry
10:42
Unanticipated N- and O- Glycopeptides
3:31
Optimization of a Label-Free Peptide Quantitation Strategy for Analysis of Therapeutic Proteins
6:25
Comparative Analysis of Herceptin N-Linked Glycosylation by HILIC-FLD and LC- MS/MS Methods
4:16
Advanced Techniques in Protein Glycosylation Analysis Using Mass Spectrometry
6:26
A Novel in vitro Serum Stability Assay for Antibody Therapeutics incorporating Internal Standards
6:56
Accurate prediction of antibody deamidations by combining high-throughput automated peptide mapping and pre-trained protein language model-based deep learning
10:23
Utilization of Intact Reconstruction to Characterize Bi-specific Antibodies
3:03

Insights

Insights 1: Modification Fine Control in Byonic™ (和訳版)
Insights 2: Quantifying Aspartic Acid Isomerization in Byos® (和訳版)
Insights 3: Intact Deconvolution with Isotopic Resolution, Intact Reconstruction in Byos® (和訳版)
Insights 4: Dynamic Columns, XIC Area Summed isoX Normalized in Byos® (和訳版)
Insights 5: MS1 Correlation Colors (和訳版)
Insights 6: Cleavage Flags in Byonic™ (和訳版)
Insights 7: Reporting Basics in Byos® (和訳版)
Insights 8: Mapping In Silico Peptides, Intact Monoisotopic Mass Visualization in Byos®
Insights 9: Delta Mod Score for Variant Analyses in Byos® and Byonic™
Insights 10: Intact Mass Workflows, Automated Annotations in Deconvoluted Spectra in Byos®
Insights 11: Validating Glycopeptide Results, MSMS Filtering in Byonic™
Insights 12: Accelerating Sequence Variant Analysis with Byos® Validators
Insights 13: Charge Deconvolution Using Know Mass Delta
Insights 14: Detached Glycan
Insights 15: Multi-Attribute Method (MAM)
Insights 16: Peptide Manager
Insights 17: Controlling how protein homology is represented in Byos
Insights 18: Using Metadata and Queries Byosphere®
Insights 19: Native MS Deconvolution
Insights 20: Glycan Cartoons in Byos
Insights 21: Investigating Unknown Mass Peaks in Byos
Insights 22: Statistics in Reports
Insights 23: Oligonucleotide Building Blocks Basics
Insights 24: Quantification of Modifications in the Peptide Workflows
Insights 25: Oligonucleotide and Impurity UV-MS Quantitation
Insights 26: Released Glycan Fragmentation
Insights 27: Digested Oligonucleotides Workflow
new Insights 28: Fold Change Capability

Feature Flyer

new HDX Analysis
new ADC Analysis
Intact Oligonucleotide Analysis
Multi-Protein Quantitation
Reconstruction

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